仿生消化法测定猪饲料原料还原糖释放量的重复性和可加性研究
发布时间：2019-05-27 20:38
【摘要】：本试验旨在建立单胃动物仿生消化系统模拟猪饲料原料消化后还原糖释放量的测定方法,为饲料养分生物学效价的评估提供参考。还原糖释放量与上样量的线性关系研究以玉米-豆粕混合物(75%玉米+25%豆粕)为研究对象,上样量设0.2、0.4、0.6、0.8和1.0 g 5个处理,每个处理4个重复,每个重复1根消化管;方法的重复性检验以玉米-豆粕混合物、大麦、花生粕和米糠为研究对象,每个样品设3个批次,每个批次4个重复;方法的可加性检验设19个处理,其中处理1~7分别为玉米、大麦、高粱、大豆粕、花生粕、棉籽粕和米糠,处理8~19为2种或2种以上饲料原料按不同比例组合制备的12种混合饲料,每个处理4个重复,每个重复1根消化管,在仿生消化系统中模拟猪消化后测定各处理的还原糖释放量。结果表明:上样量在0.2~0.8 g时,还原糖总释放量与上样量呈显著的线性关系(R2=0.999 2),还原糖相对释放量在559.56~582.70 mg/g DM变化,变异系数为1.66%,而上样量为1.0 g时,还原糖相对释放量比上样量为0.2~0.8 g时的平均值下降5.37%;3个批次的大麦、花生粕、米糠和玉米-豆粕混合物的还原糖释放量的批内变异系数、批间变异系数和总变异系数均不大于1.68%,批间最大相对偏差分别为0.68%、1.50%、1.39%和0.29%;12种混合饲料还原糖释放量的实测值显著高于计算值(P0.05),而还原糖释放量的计算值与实测值的线性回归模型与y=x相重合(截距P=0.480 5;斜率P=0.514 1)。由此得出,当上样量在0.2~0.8 g时,上样量与还原糖释放量呈显著线性关系;仿生消化法测定猪饲料原料还原糖释放量的重复性和可加性满足定量分析的基本要求。
[Abstract]:The purpose of this study was to establish a method for the determination of reducing sugar release from pig feed after digestion by bionic digestive system of monogastric animals, and to provide a reference for the evaluation of the biological titer of feed nutrients. The linear relationship between reduced sugar release and sampling volume was studied by using corn-soybean meal mixture (75% corn 25% soybean meal) as the research object. The sample size was 0.2, 0.4, 0.6, 0.8 and 1.0 g, respectively, with 4 repeats in each treatment. One digestive tube per repetition; The reproducibility test of corn-soybean meal mixture, barley, peanut meal and rice husk was carried out. Each sample was divided into 3 batches and 4 repeats per batch. The additivity of the method was determined by 19 treatments, including corn, barley, sorghum, soybean meal, peanut meal, cottonseed meal and rice husk, respectively. Treatment 8 / 19 is 12 kinds of mixed feed prepared by combination of 2 or more feed materials in different proportion, with 4 repeats per treatment and 1 digestive tube per repetition. The release of reducing sugar from each treatment was measured in bionic digestion system. The results showed that when the sample size was 0.2 鈮，
本文编号：2486462
[Abstract]:The purpose of this study was to establish a method for the determination of reducing sugar release from pig feed after digestion by bionic digestive system of monogastric animals, and to provide a reference for the evaluation of the biological titer of feed nutrients. The linear relationship between reduced sugar release and sampling volume was studied by using corn-soybean meal mixture (75% corn 25% soybean meal) as the research object. The sample size was 0.2, 0.4, 0.6, 0.8 and 1.0 g, respectively, with 4 repeats in each treatment. One digestive tube per repetition; The reproducibility test of corn-soybean meal mixture, barley, peanut meal and rice husk was carried out. Each sample was divided into 3 batches and 4 repeats per batch. The additivity of the method was determined by 19 treatments, including corn, barley, sorghum, soybean meal, peanut meal, cottonseed meal and rice husk, respectively. Treatment 8 / 19 is 12 kinds of mixed feed prepared by combination of 2 or more feed materials in different proportion, with 4 repeats per treatment and 1 digestive tube per repetition. The release of reducing sugar from each treatment was measured in bionic digestion system. The results showed that when the sample size was 0.2 鈮，
本文编号：2486462
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