葛根芩连汤防治小鼠感染大肠杆菌的作用观察

发布时间：2019-05-29 18:58

【摘要】：为了观察葛根芩连汤对感染大肠杆菌小鼠的防治作用,试验采用将葛根芩连汤制成浓度为1 g/mL、0.5 g/mL、0.25 g/mL的药液,分别对小鼠进行灌胃,试验分为治疗试验和预防试验,记录攻毒后48 h内小鼠的死亡数,并计算其死亡率;同时于攻毒前(0小时)和攻毒后第6小时、12小时、18小时、24小时和48小时用温度传感器检测各组小鼠肛门周围的体表温度。结果表明:在预防试验中,预防高剂量组小鼠的存活率达到80%;在治疗试验中,治疗高剂量组小鼠的死亡率极显著低于阴性对照组(P0.01),治疗中剂量组小鼠的死亡率显著低于阴性对照组(P0.05)。在预防试验第6,12小时,预防高、中、低剂量组小鼠的体温与阴性对照组相比差异显著(P0.05);在治疗试验第6,18小时,治疗高、中、低剂量组小鼠的体温与阴性对照组相比差异显著(P0.05)。说明葛根芩连汤对感染大肠杆菌的小鼠具有较好的预防和治疗效果。
[Abstract]:In order to observe the preventive and therapeutic effect of Gegen Qinlian decoction on mice infected with Escherichia coli, the mice were treated with Gegen Qinlian decoction at the concentration of 1 g 路mL, 0.5 g / mL and 0.25 g/mL, respectively. The experiment was divided into treatment test and prevention test. The death rate of mice within 48 hours after challenge was recorded, and the mortality rate was calculated. At the same time, the body surface temperature around anus of mice in each group was measured by temperature sensor before (0 hours) and 6 hours, 12 hours, 18 hours, 24 hours and 48 hours after challenge. The results showed that the survival rate of mice in the high dose group was 80%. In the treatment test, the mortality of the high dose group was significantly lower than that of the negative control group (P 0.01), and the mortality of the middle dose group was significantly lower than that of the negative control group (P 0.05). At the 6th and 12th hours of the prevention test, the body temperature of the mice in the high, middle and low dose groups was significantly different from that in the negative control group (P 0.05). At the 6th and 18th hours of the treatment trial, the body temperature of the mice in the high, middle and low dose groups was significantly different from that in the negative control group (P 0.05). The results showed that Gegen Qinlian decoction had a good preventive and therapeutic effect on mice infected with Escherichia coli.
【作者单位】：青岛农业大学;
【基金】：国家科技支撑计划项目(2011BAD34B03)
【分类号】：S853.74

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