广东一株牛源流行性出血病病毒的分离鉴定
发布时间：2019-06-05 14:48
【摘要】：为了解广东反刍动物流行性出血病(EHD)的感染情况,本研究对采集的牛血液样品进行EHD病毒(EHDV)的RT-PCR检测,对核酸检测为阳性的样品进行病毒分离鉴定。将阳性样品接种BHK-21细胞连续传3代时出现细胞病变,扩增出了VP7基因,进行BLAST序列比对,结果表明该分离株与国内外EHDV分离株的同源性达98%~100%,TCID_(50)为10~(-6.5)/50μL,不能凝集鸡红细胞,表明该分离株为EHDV,命名为GD-N133。根据VP2基因序列的分析和微量中和试验结果,确定该病毒株为EHDV-1型。
[Abstract]:In order to understand the infection of epidemic hemorrhagic disease (EHD) in ruminants in Guangdong, the RT-PCR of EHD virus (EHDV) was detected in bovine blood samples, and the virus was isolated and identified in the samples with positive nucleic acid. The positive samples were inoculated with BHK-21 cells for three consecutive generations, and the VP7 gene was amplified and compared with BLAST sequences. the results showed that the homology between the isolate and EHDV isolates at home and abroad was 98% 鈮，
本文编号：2493604
[Abstract]:In order to understand the infection of epidemic hemorrhagic disease (EHD) in ruminants in Guangdong, the RT-PCR of EHD virus (EHDV) was detected in bovine blood samples, and the virus was isolated and identified in the samples with positive nucleic acid. The positive samples were inoculated with BHK-21 cells for three consecutive generations, and the VP7 gene was amplified and compared with BLAST sequences. the results showed that the homology between the isolate and EHDV isolates at home and abroad was 98% 鈮，
本文编号：2493604
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