高通量测序技术分析大骨鸡盲肠微生物组成

发布时间：2019-06-05 23:39

【摘要】：研究旨在利用高通量测序技术比较散养(OD)和笼养(ID)两种饲养方式下12、18周龄大骨鸡盲肠微生物组成差异。结果表明,随着日龄的增加,OD组操作分类单位(OTUs)数量变化不大,而ID组OTUs数量有所增加。从门的丰富度来看,各组中拟杆菌门最丰富,比例最大,其次为厚壁菌门和变形菌门;12周龄时,散养组与笼养组间有3个菌门差异显著(P0.05),18周龄时,有5个菌门差异显著(P0.05);在属的水平,共检测到60个属,12周龄时,散养组与笼养组间有10个属的含量存在显著差异(P0.05),18周龄时,散养组与笼养组间有6个属的含量存在显著差异(P0.05)。研究结果为大骨鸡养殖策略的制定及盲肠微生物区系的研究提供参考。
[Abstract]:The purpose of this study was to compare the microbial composition of cecum of 18-week-old big bone chickens under 12 weeks old (OD) and captive (ID) by high throughput sequencing. The results showed that with the increase of age, the number of operational classification units (OTUs) in OD group changed little, while the number of OTUs in ID group increased. From the point of view of the richness of the door, the bacteria were the most abundant and the proportion was the largest in each group, followed by the thick-wall phylum and Proteus. At the age of 12 weeks, there were significant differences in three bacteria between the free culture group and the cage culture group (P 0.05), and at the age of 18 weeks, there were 5 significant differences between the two groups (P 0.05). At the level of genus, a total of 60 genus were detected. At the age of 12 weeks, there was a significant difference in the content of 10 genus between the free culture group and the cage group (P 0.05). At the age of 18 weeks, there was a significant difference in the content of 10 genus between the free culture group and the cage group (P 0.05). There were significant differences in the contents of 6 genus between the free culture group and the cage culture group (P 0.05). The results provide a reference for the formulation of breeding strategy and the study of cecum microflora.
【作者单位】：锦州医科大学食品科学与工程学院;锦州医科大学畜牧兽医学院;
【基金】：辽宁省自然科学基金项目(2014022046)
【分类号】：S831

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