一起山羊流产衣原体感染的诊断

发布时间：2019-06-15 12:22

【摘要】：陕西省某山羊养殖场发生了一起山羊流产病,经流行病学调查,临床症状和病理剖检观察进行初步诊断;利用布鲁菌虎红平板凝集试验、衣原体间接血凝试剂盒对10份流产母羊血清检测;对病料进行细菌学检测;依据GenBank收录的山羊流产性衣原体基因组设计1对特异性引物,通过PCR方法从病料中扩增衣原体特异性片段。结果表明,根据临床症状和病理变化初步怀疑为布鲁菌和鹦鹉热亲衣原体感染;10份血清检测结果为布鲁菌病血清全部阴性,衣原体感染血清全部为阳性;细菌学检测结果为阴性;PCR结果获得523bp基因片段,测序结果与鹦鹉热亲衣原体100%相似。依据流行病学调查,临床症状和病理剖检观察,病原学和血清学诊断,最终确诊该病为鹦鹉热亲衣原体引起的山羊地方流行性流产。
[Abstract]:A goat abortion disease occurred in a goat farm in Shaanxi Province, which was diagnosed by epidemiological investigation, clinical symptoms and pathological examination, 10 aborted ewe serum were detected by brucellosis tiger red plate agglutination test, 10 aborted ewes were detected by chlamydia indirect hemagglutination kit, and bacteriological examination was carried out on the diseased materials. According to the genome of goat chlamydia abortion collected by GenBank, a pair of specific primers were designed, and the specific fragments of chlamydia were amplified from the diseased materials by PCR. The results showed that the infection of brucellosis and chlamydia parrot was suspected according to the clinical symptoms and pathological changes. The results of 10 serum samples showed that all the serum of brucellosis was negative and the serum of chlamydia infection was all positive. The results of bacteriological test were negative. The 523bp gene fragment was obtained by PCR results, and the sequencing results were 100% similar to those of chlamydia parrot. According to epidemiological investigation, clinical symptom and pathological examination, etiology and serological diagnosis, the disease was finally diagnosed as local epidemic abortion caused by chlamydia parrot.
【作者单位】：西北农林科技大学动物医学院;安徽农业大学动物科技学院;
【基金】：陕西省农业科技创新与攻关项目(2016NY-092) 陕西省科技统筹创新工程计划项目(2016KTZDNY02-05) 杨凌示范区农业科技示范推广项目(TS-2016-13;2017-TS-23)
【分类号】：S858.27

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