国内部分地区样品猪流感H1、H3感染状况调查分析

发布时间：2019-06-22 14:52

【摘要】：本实验的目的是为了调查国内部分地区样品猪流感H1N1、H3N2的感染流行状况,根据检测国内部分猪场猪流感H1N1、H3N2的抗体阳性率,为国内猪场评估养殖场H1N1、H3N2感染状况,及是否需要进行免疫提供一定的决策依据。鉴于猪流感在养殖场仍属于非疫苗免疫疫病的情况,本实验运用ELISA检测方法检测血清抗体作为感染指标。采用的是上海研吉生物有限公司分包装的ELISA试剂盒,对2011年~2012年上海地区以及安徽、河南、山东、福建等外省市地区的24个场次共计365份血清进行了猪流感H1N1、H3N2抗体阳性的检测。实验检测结果如下:365份血样中,猪流感H1N1的抗体阳性数为113份,阳性率为30.96%;H3N2抗体阳性数54份,阳性率为14.79%。不同阶段的育肥猪、种猪中的315份血样检测中H1N1、H3N2抗体数分别为99份、46份,抗体阳性率分别为31.43%和14.60%,母猪50份血样中H1N1、H3N2抗体数分别为13份、7份,抗体阳性率分别为26.00%和14.00%;对不同地区的猪只进行检测,其中上海地区273份,H1N1、H3N2抗体阳性数分别为76份、26份,抗体阳性率分别为27.84%%和9.52%;外省市地区92份,H1N1、H3N2抗体阳性数分别为36份、27份,抗体阳性率分别为39.13%和29.35%;对不同周龄的猪只检测,其中9周龄的猪只相比其他3、5、7周龄来说,H1N1、H3N2抗体阳性率较高的;在不同月份对猪只进行检测,3月份检测224份H1N1抗体阳性数87份,阳性率38.84%,H3N2抗体阳性数41份,阳性率18.30%;4月份检测50份H1N1抗体阳性数3份,阳性率6.00%,H3N2抗体阳性数1份,阳性率2.00%;11月份检测91份H1N1抗体阳性数22份,阳性率24.18%,H3N2抗体阳性数11份,阳性率12.09%。365份血清中同时检测出H1N1、H3N2抗体阳性的为43份,阳性率为11.78%。根据试验的结果可得出初步结论,国内部分猪流感H1N1抗体阳性率维持在一定水平,H3N2的抗体阳性率比H1N1相对较低,P0.05,差异显著。上海地区的猪流感H1N1、H3N2的抗体阳性率相比外省市地区略低,P0.05,差异显著。在各个不同季节中春季是猪流感的高发期,9周龄的猪更易感染流感。
[Abstract]:The purpose of the experiment is to investigate the prevalence of swine influenza A (H1N1) and H3N2 in the domestic part of the pig farm, and to evaluate the infection status of H1N1 and H3N2 in the domestic pig farm according to the positive rate of the antibody to the swine influenza H1N1 and H3N2 in the domestic pig farm. And whether to provide certain decision-making basis for immunization. In view of the fact that the swine flu still belongs to the non-vaccine immune disease in the farm, this experiment uses the ELISA method to detect the serum antibody as the infection index. A total of 365 sera from 24 fields in Shanghai and other provinces, such as Anhui, Henan, Shandong, Fujian and other provinces in Shanghai and other provinces and cities in Anhui, Henan, Shandong and Fujian were used to detect the positive of swine influenza A (H1N1) and H3N2 antibody. The results of the experiment were as follows: in 365 blood samples, the number of antibody positive in swine influenza H1N1 was 113, the positive rate was 30.96%, and the positive rate of H3N2 antibody was 54, and the positive rate was 14.79%. The positive rates of H1N1 and H3N2 were 31.43% and 14.60%, respectively. The number of H1N1 and H3N2 in 50 samples of the sow was 13,7, and the positive rate of the antibody was 26.00% and 14.00%, respectively. The results showed that the positive rates of the antibodies in 273, H1N1 and H3N2 in Shanghai area were 76,26 and 9.52%, respectively. The positive rates of the antibodies in 92, H1N1 and H3N2 were 36 and 27, respectively. The positive rate of the antibody was 39.13% and 29.35%, respectively; for pigs of different age, the positive rate of H1N1 and H3N2 was higher than that of the other 3,5 and 7 weeks of age; in different months, the positive rate of the H1N1 and H3N2 antibodies was higher; in the same month, only the detection of the pigs was carried out, and the positive rate of 224 cases of H1N1 antibody was detected in March, and the positive rate was 38.84%. The positive rate of H3N2 antibody was 41, the positive rate was 18.30%; in April, the positive number of 50 H1N1 antibodies was detected in 3, the positive rate was 6.00%, the positive rate of H3N2 antibody was 1, the positive rate was 2.00%; in November, the positive rate of 91 H1N1 antibody was 22, the positive rate was 24.18%, and the positive rate of H3N2 antibody was 11. The positive rate was 12.09%. The positive rate of H1N1 and H3N2 was detected in 365 sera, and the positive rate was 11.78%. According to the results of the test, it is concluded that the positive rate of the swine influenza A (H1N1) antibody in the domestic part is kept at a certain level, and the positive rate of the antibody of H3N2 is lower than that of H1N1 (P0.05). The positive rate of the antibody of swine influenza (H1N1) and H3N2 in the Shanghai area was slightly lower than that in the provinces and cities (P0.05). In each of the different seasons, the spring is the high-onset of the swine flu, and the 9-week-old pig is more likely to be infected with the flu.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28

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