狐肺炎大肠杆菌HtrA蛋白原核表达及多抗血清制备
发布时间：2019-06-24 14:15
【摘要】：利用PCR方法从银狐大肠杆菌基因组中克隆出htra基因,测序无误后将其构建到pET32a原核表达载体上,重组载体转化BL21大肠杆菌诱导重组蛋白表达,通过SDS-PAGE和Western blot方法验证重组蛋白得到很好表达;用亲和层析方法纯化蛋白,纯化的蛋白免疫小鼠,所得的多抗血清能够特异性识别大肠杆菌HtrA蛋白,为研究HtrA蛋白的作用机制奠定基础。
[Abstract]:The hra gene was cloned from the genome of the E. coli. The recombinant vector was transformed into E. coli to induce the expression of the recombinant protein, and the recombinant protein was expressed by SDS-PAGE and Western blot. The purified protein was purified by affinity chromatography, and the purified protein was used to immunize the mouse. The obtained polyclonal antiserum can specifically identify the E. coli HtrA protein and lay the foundation for studying the mechanism of the action of the HtrA protein.
【作者单位】： 河北科技师范学院河北省预防兽医学重点实验室;秦皇岛第二医院;
【基金】：国家“星火计划”资助项目(2015GA620002) 河北省科技厅“科技支撑计划”资助项目(14826613D) 河北科技师范学院博士启动基金资助项目(2015YB002) 河北省高等学校科学技术研究资助项目(ZD2016067) 秦皇岛市农业科学研究院资助项目(2014-04)
【分类号】：S852.61
，
本文编号：2505124
[Abstract]:The hra gene was cloned from the genome of the E. coli. The recombinant vector was transformed into E. coli to induce the expression of the recombinant protein, and the recombinant protein was expressed by SDS-PAGE and Western blot. The purified protein was purified by affinity chromatography, and the purified protein was used to immunize the mouse. The obtained polyclonal antiserum can specifically identify the E. coli HtrA protein and lay the foundation for studying the mechanism of the action of the HtrA protein.
【作者单位】： 河北科技师范学院河北省预防兽医学重点实验室;秦皇岛第二医院;
【基金】：国家“星火计划”资助项目(2015GA620002) 河北省科技厅“科技支撑计划”资助项目(14826613D) 河北科技师范学院博士启动基金资助项目(2015YB002) 河北省高等学校科学技术研究资助项目(ZD2016067) 秦皇岛市农业科学研究院资助项目(2014-04)
【分类号】：S852.61
，
本文编号：2505124
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