黑龙江省全沟硬蜱携带伯氏疏螺旋体广义种和斑点热群立克次体的研究
发布时间：2019-07-17 14:49
【摘要】：为了解黑龙江省牡丹江市、鹤岗市萝北县、哈尔滨市通河县全沟硬蜱携带伯氏疏螺旋体和斑点热群立克次体的情况,在获得25只全沟硬蜱细菌组学数据的基础上,通过PCR扩增16S r RNA和fla基因检测伯氏疏螺旋体,扩增glt A和r Omp A基因检测斑点热群立克次体。结果显示,伯氏疏螺旋体的阳性率为20.0%(5/25),斑点热群立克次体的阳性率为68.0%(17/25)。另外,伯氏疏螺旋体阳性样品均显示斑点热群立克次体阳性,这两种病原的混合感染率为20.0%(5/25)。序列分析结果显示,16S r RNA基因与伽氏疏螺旋体Nov405菌株的相似性为99.65%,fla基因与伽氏疏螺旋体SZ菌株的相似性为99.73%;glt A和r Omp A基因与塔拉塞维奇立克次体暂定株(Candidatus Rickettsia tarasevichiae)的相似性均为100%。结果表明,斑点热群立克次体(Candidatus Rickettsia tarasevichiae)和伯氏疏螺旋体广义种在全沟硬蜱中广泛存在,这两种病原的混合感染增加了蜱传人兽共患病的风险。
[Abstract]:In order to understand the carrying of Borrelia burgdorferi and spotted fever group rickettsia in Mudanjiang City, Hegang City, Luobei County, Harbin City and Tonghe County, Harbin City, on the basis of obtaining the bacteriological data of 25 ticks, the 16s r RNA and fla genes were amplified by PCR, and the dot fever group rickettsia was detected by glt A and r Omp A genes. The results showed that the positive rate of Borrelia burgdorferi was 20.0% (5 鈮，
本文编号：2515502
[Abstract]:In order to understand the carrying of Borrelia burgdorferi and spotted fever group rickettsia in Mudanjiang City, Hegang City, Luobei County, Harbin City and Tonghe County, Harbin City, on the basis of obtaining the bacteriological data of 25 ticks, the 16s r RNA and fla genes were amplified by PCR, and the dot fever group rickettsia was detected by glt A and r Omp A genes. The results showed that the positive rate of Borrelia burgdorferi was 20.0% (5 鈮，
本文编号：2515502
本文链接：https://www.wllwen.com/yixuelunwen/dongwuyixue/2515502.html
