湖北省儿童和乳牛中隐孢子虫和贾第虫的分子流行病学研究
发布时间：2021-03-04 10:57
隐孢子虫（Cryptosporidium）和贾第虫（Giardia）是两种重要的人畜共患肠道原虫,它们通常是经过粪-口途径直接进入人或动物的宿主体内,伴随着卵囊的摄取,最终引起人和动物的隐孢子虫病和贾第虫病,并伴随着宿主的腹泻;最近的流行病学研究表明,在隐孢子虫病和贾第虫病中,人畜共患之间的传播起着重要的作用;牛,尤其是乳牛,是作为隐孢子虫病和贾第虫病在人和动物之间传播的重要来源。然而,在一些地区,例如中国湖北省,关于人和犊牛的隐孢子虫和贾第虫的流行病学的数据（比如优势虫种和基因型）却仍是一片空白。为此,本文针对这些问题开展了如下的研究:在第1章中,对这两种原生寄生虫的背景知识、关键的物种、生活史、传播途径、病原体的发病机制、诊断及其防控进行详细的介绍;并总结了已有的物种和基因型。通过对分子流行病学调查的总结,提供了对隐孢子虫和贾第虫病防控的研究方向。在第2章中,对湖北省武汉市腹泻儿童的隐孢子虫、贾第虫和微孢子虫的分子流行病学调查进行了首次报道。在2016年6月至2016年8月,对武汉市儿童医院和武汉大学人民医院门诊部的腹泻儿童新鲜粪便样品共计500份进行了隐孢子虫、贾第虫和微孢子虫的...
【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校
【文章页数】：161 页
【学位级别】：硕士
【文章目录】：
Abstract
中文摘要
CHAPTER 1 - LITERATURE REVIEW
1.1. Introduction
1.2. Historical background and significance
1.2.1. Cryptosporidium
1.2.2. Giardia
1.3. Cryptosporidium taxonomy and classification
1.3.1. Nomenclature history
1.3.2. Cryptosporidium species
1.3.2.1 Cryptosporidium species of mammals
1.3.2.2 Cryptosporidium species of birds
1.3.2.3 Cryptosporidium species of reptiles
1.3.2.4 Cryptosporidium species of fish
1.3.2.5 Cryptosporidium species of amphibians
1.3.3. Giardia classification
1.4. General life cycles
1.4.1. Cryptosporidium species
1.4.2. Life cycle of Giardia
1.5. Pathogenesis
1.6. Clinical manifestations
1.7. Diagnosis
1.7.1 Conventional diagnosis
1.7.1.1 Direct microscopy
1.7.1.2 Flotation methods
1.7.1.3 Staining techniques
1.7.2. Immunological tools
1.7.3. Molecular tools
1.7.3.1 Nested-PCR
1.7.3.2 RFLP analysis
1.7.3.3 Real-time PCR
1.7.3.4 Multiplexed tandem-PCR （MT-PCR）
1.7.3.5 Other PCR methods
1.8. Epidemiology
1.8.1. Molecular epidemiology of Cryptosporidium species and genotypes in humans
1.8.2. Cryptosporidium parvum subtypes and zoonotic transmission
1.8.2.1 Cryptosporidium parvum subtypes in humans, and zoonotic transmission
1.8.2.2. Cryptosporidium parvum subtypes in animals
1.8.3 Epidemiology of Giardia
1.8.3.1 Zoonotic potential of Giardia
1.8.4. Epidemiological evidence for zoonotic cryptosporidiosis and giardiasis
1.8.4.1 Exposure
1.8.4.2 Routes of transmission
1.9. Recent insights into Cryptosporidium of cattle in China
1.9.1. Cryptosporidium species and subtypes in dairy cattle in China
1.9.2. Cryptosporidium species and subtypes in yaks in China
1.9.3. Cryptosporidium species/subtypes in beef cattle and water buffalo in China
1.9.4. Age distributions of Cryptosporidium species in cattle
1.10. Recent insights into Cryptosporidium of humans in China
1.11. Aspects of treatment, prevention, and control
1.12. Conclusions from the literature and future prospects
CHAPTER 2 - FIRST SURVEY OF CRYPTOSPORIDIUM, GIARDIA AND ENTEROCYTOZOON IN DIARRHORIC CHILDREN FROM WUHAN, CHINA ..
2.1. Introduction
2.2. Materials and methods
2.3. Results and discussion
2.3.1. Cryptosporidium
2.3.2. Giardia
2.3.3. Enterocytozoon
2.4. Concluding remarks
CHAPTER 3 - MOLECULAR INVESTIGATION OF CRYPTOSPORIDIUM AND GIARDIA FROM PRE- AND POST-WEANED CALVES IN HUBEI PROVINCE ..
3.1. Introduction
3.2. Materials and methods
3.3. Results and discussion
3.3.1. Cryptosporidium
3.3.2. Giardia
3.4. Conclusions
CHAPTER 4 - GENERAL DISCUSSION
Conclusion
References
ACKNOWLEDGEMENTS
CURRICULUM VITAE
【参考文献】：
期刊论文
[1]上海市综合性医院腹泻患者隐孢子虫感染状况调查[J]. 张小萍,何艳燕,王真瑜,张耀光,朱倩,蒋守富,李颖,成玉萍,尹明敏. 中国热带医学. 2016(12)
[2]四川红原县牦牛隐孢子虫感染情况分子流行病学调查[J]. 郝力力,李锐,段玲,贺亮. 浙江农业学报. 2016(11)
[3]我国部分农村地区儿童隐孢子虫感染现况调查[J]. 王丽,田向红,何祖安,何昌云,丁震,夏云婷,查玉娥,张荣. 环境卫生学杂志. 2015(04)
[4]安徽省局部地区人群隐孢子虫感染情况调查[J]. 王庆权,郭见多,操治国,汪奇志,刘道华,汪天平. 中国血吸虫病防治杂志. 2015(03)
[5]牧区儿童隐孢子虫感染情况及危险因素的调查[J]. 孙玉荣. 中国卫生产业. 2015(08)
[6]2011-2013年上海临床医院送检样品寄生虫检测结果分析[J]. 陈韶红,张永年,李浩,蔡玉春,陈家旭. 中国寄生虫学与寄生虫病杂志. 2014(06)
[7]广州市哨点医院腹泻儿童隐孢子虫感染的分子流行病学研究[J]. 姚月娴,陈惠芳,刘谢,效拟,肖媛,黄燕惠,赵德坚,陈清,俞守义. 热带医学杂志. 2014(01)
[8]湖北赤壁市农村儿童隐孢子虫感染情况[J]. 何祖安,付立新,周文珊,陈娇,田炳秋,王丽,张荣. 公共卫生与预防医学. 2013(06)
[9]兰州市慢性病患者隐孢子虫和溶组织阿米巴感染流行病学调查结果分析[J]. 王雁南,董甜甜,王爱秀,党文,逯召莲,张思,陈根. 中国病原生物学杂志. 2013(10)
[10]广西奶水牛场隐孢子虫感染情况的调查[J]. 谢永平,魏晓瑞,李军,陶立,闭炳芬,韦志锋,彭昊,陈泽祥,杨威,王晓丽. 动物医学进展. 2013(06)
本文编号：3063093
【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校
【文章页数】：161 页
【学位级别】：硕士
【文章目录】：
Abstract
中文摘要
CHAPTER 1 - LITERATURE REVIEW
1.1. Introduction
1.2. Historical background and significance
1.2.1. Cryptosporidium
1.2.2. Giardia
1.3. Cryptosporidium taxonomy and classification
1.3.1. Nomenclature history
1.3.2. Cryptosporidium species
1.3.2.1 Cryptosporidium species of mammals
1.3.2.2 Cryptosporidium species of birds
1.3.2.3 Cryptosporidium species of reptiles
1.3.2.4 Cryptosporidium species of fish
1.3.2.5 Cryptosporidium species of amphibians
1.3.3. Giardia classification
1.4. General life cycles
1.4.1. Cryptosporidium species
1.4.2. Life cycle of Giardia
1.5. Pathogenesis
1.6. Clinical manifestations
1.7. Diagnosis
1.7.1 Conventional diagnosis
1.7.1.1 Direct microscopy
1.7.1.2 Flotation methods
1.7.1.3 Staining techniques
1.7.2. Immunological tools
1.7.3. Molecular tools
1.7.3.1 Nested-PCR
1.7.3.2 RFLP analysis
1.7.3.3 Real-time PCR
1.7.3.4 Multiplexed tandem-PCR （MT-PCR）
1.7.3.5 Other PCR methods
1.8. Epidemiology
1.8.1. Molecular epidemiology of Cryptosporidium species and genotypes in humans
1.8.2. Cryptosporidium parvum subtypes and zoonotic transmission
1.8.2.1 Cryptosporidium parvum subtypes in humans, and zoonotic transmission
1.8.2.2. Cryptosporidium parvum subtypes in animals
1.8.3 Epidemiology of Giardia
1.8.3.1 Zoonotic potential of Giardia
1.8.4. Epidemiological evidence for zoonotic cryptosporidiosis and giardiasis
1.8.4.1 Exposure
1.8.4.2 Routes of transmission
1.9. Recent insights into Cryptosporidium of cattle in China
1.9.1. Cryptosporidium species and subtypes in dairy cattle in China
1.9.2. Cryptosporidium species and subtypes in yaks in China
1.9.3. Cryptosporidium species/subtypes in beef cattle and water buffalo in China
1.9.4. Age distributions of Cryptosporidium species in cattle
1.10. Recent insights into Cryptosporidium of humans in China
1.11. Aspects of treatment, prevention, and control
1.12. Conclusions from the literature and future prospects
CHAPTER 2 - FIRST SURVEY OF CRYPTOSPORIDIUM, GIARDIA AND ENTEROCYTOZOON IN DIARRHORIC CHILDREN FROM WUHAN, CHINA ..
2.1. Introduction
2.2. Materials and methods
2.3. Results and discussion
2.3.1. Cryptosporidium
2.3.2. Giardia
2.3.3. Enterocytozoon
2.4. Concluding remarks
CHAPTER 3 - MOLECULAR INVESTIGATION OF CRYPTOSPORIDIUM AND GIARDIA FROM PRE- AND POST-WEANED CALVES IN HUBEI PROVINCE ..
3.1. Introduction
3.2. Materials and methods
3.3. Results and discussion
3.3.1. Cryptosporidium
3.3.2. Giardia
3.4. Conclusions
CHAPTER 4 - GENERAL DISCUSSION
Conclusion
References
ACKNOWLEDGEMENTS
CURRICULUM VITAE
【参考文献】：
期刊论文
[1]上海市综合性医院腹泻患者隐孢子虫感染状况调查[J]. 张小萍,何艳燕,王真瑜,张耀光,朱倩,蒋守富,李颖,成玉萍,尹明敏. 中国热带医学. 2016(12)
[2]四川红原县牦牛隐孢子虫感染情况分子流行病学调查[J]. 郝力力,李锐,段玲,贺亮. 浙江农业学报. 2016(11)
[3]我国部分农村地区儿童隐孢子虫感染现况调查[J]. 王丽,田向红,何祖安,何昌云,丁震,夏云婷,查玉娥,张荣. 环境卫生学杂志. 2015(04)
[4]安徽省局部地区人群隐孢子虫感染情况调查[J]. 王庆权,郭见多,操治国,汪奇志,刘道华,汪天平. 中国血吸虫病防治杂志. 2015(03)
[5]牧区儿童隐孢子虫感染情况及危险因素的调查[J]. 孙玉荣. 中国卫生产业. 2015(08)
[6]2011-2013年上海临床医院送检样品寄生虫检测结果分析[J]. 陈韶红,张永年,李浩,蔡玉春,陈家旭. 中国寄生虫学与寄生虫病杂志. 2014(06)
[7]广州市哨点医院腹泻儿童隐孢子虫感染的分子流行病学研究[J]. 姚月娴,陈惠芳,刘谢,效拟,肖媛,黄燕惠,赵德坚,陈清,俞守义. 热带医学杂志. 2014(01)
[8]湖北赤壁市农村儿童隐孢子虫感染情况[J]. 何祖安,付立新,周文珊,陈娇,田炳秋,王丽,张荣. 公共卫生与预防医学. 2013(06)
[9]兰州市慢性病患者隐孢子虫和溶组织阿米巴感染流行病学调查结果分析[J]. 王雁南,董甜甜,王爱秀,党文,逯召莲,张思,陈根. 中国病原生物学杂志. 2013(10)
[10]广西奶水牛场隐孢子虫感染情况的调查[J]. 谢永平,魏晓瑞,李军,陶立,闭炳芬,韦志锋,彭昊,陈泽祥,杨威,王晓丽. 动物医学进展. 2013(06)
本文编号：3063093
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