猪卵母细胞单精子胞浆内注射胚胎的体外生产与发育
发布时间：2021-09-02 01:05
蛋白质供应是马拉维以及整个非洲最重要的问题之一。马拉维国土面积小,养猪业尚处于初级阶段,但发展潜力巨大。全国生猪存栏量超过3,000,000头,主要集中于农村。养猪业对于农村和城市都是重要的经济产业。猪肉消费量、生猪交易量和有机肥料是评价当地养猪业的重要指标。马拉维政府重视养猪生产,并致力于提高生猪饲养、育种和疫病控制,促进养猪生产。为提高胴体品质、产仔数和肉品数量,还开展了与外来品种的杂交育种工作。政府对非洲猪瘟等疾病进行严格防控。政策上鼓励肉品生产,控制危害人类健康的疾病,养猪主要供当地消费,剩余的用来出口。在马拉维,相比其他家畜对当地猪繁殖性能的研究很少。当地猪群因某些疫病的周期性暴发而减少,如非洲猪瘟。另一个挑战是体内外寄生虫病的爆发。这些疾病的发生会影响到各年龄段生猪的生产性能和繁殖性能,造成局部地区甚至全国范围的经济损失。猪的繁殖能力高,每年可产多胎；但有关动物遗传资源保护的资料缺乏。在马拉维,与移地保护相对应的是就地保护。研究发现移地保护更加可行,主要方法包括猪精液、卵母细胞以及胚胎的玻璃化冷冻。玻璃化冷冻即在液氮中保存精子、卵母细胞或胚胎的方法,可用于濒临灭绝的动物资源...
【文章来源】：南京农业大学江苏省 211工程院校 教育部直属院校
【文章页数】：94 页
【学位级别】：硕士
【文章目录】：
摘要
ABSTRACT
Part 1:Literature Review
Chapter 1:GENERAL SITUATION ON EMBRYO PRODUCTION IN FARM ANIMALS
1.1 SUCCESS OF EMBRYO PRODUCTION BY ICSI METHOD IN PIG AND OTHER FARM ANIMALS
1.2 ICSI PROCESS AND ELECTRIC ACTIVATION OF OOCYTES
1.3 THE STATUS OF IN VITRO FERTILIZATION TECHNIQUE IN EMBRYO PRODUCTION
1.4 THE PROSPECTS OF SOMATIC CELL NUCLEAR TRANSFER
1.4.1 SELECTION OF DONOR CELLS
1.4.2 ENUCLEATION OF RECIPIENT CELLS AND NUCLEAR TRANSFER
1.4.3 ACTIVATION
1.4.4 EMBRYO CULTURE
1.4.5 REMODELLING AND REPROGRAMMING
Part 2:EXPERIMENTAL STUDY
Chapter 2:MALAWI SITUATION ON PIG AND OTHER FARM ANIMAL GENETIC RESOURCESCONSERVATION
2.1 INTRODUCTION
2.1.1 PREVIOUS AND ONGOING EFFORTS BY OTHER SCIENTISTS IN MALAWI
2.1.2 CROSSBREEDING PROGRAMME
2.1.3 PIGS
2.1.4 CATTLE
2.1.5 SMALL RUMINANTS
2.1.6 CHICKENS
2.2 SELECTION
2.2.1 CATTLE
2.2.2 SMALL RUMINANTS
2.2.3 POULTRY
2.2.4 PIGS
2.3 CURRENT NATIONAL STRATEGIES FOR CONSERVATION,IMPRVEMENT AND UTILIZATION OF FARM ANIMAL GENETIC RESOURCES(AnGRs)
2.3.1 BREED SURVEY AND FIELD GUIDES
2.3.2 COMMUNITY BASED LIVESTOCK ACTIVITIES
2.3.3 PROPOSALS
2.3.4 NATIONAL BIODIVERSITY STRATEGIES ACTION PLAN
2.3.5 CONSTRAITS IN GENETIC IMPROVEMENT PROGRAMMES
2.3.6 MALAWI LOCAL PIG BREED
2.3.7 PIG NUMBERS AND OWNERSHIP PATTERN
2.4 THE VALUE OF LOCAL PIGS AMONG SMALLHOLDER FARMERS IN MALAWI
2.4.1 POVERTY ALLEVIATION PERSPECTIVE
2.4.2 AS SOURCE OF FOOD
2.4.3 TOLERANCE TO DISEASES AND PARASITES
2.4.4 DISASTER PREPAREDNESS
2.4.5 RESEARCH NEEDS
2.4.6 STRATEGIES TO CONSERVE LOCAL PIGS
2.4.7 THREATS TO INDIGENOUS PIGS
2.4.8 STAKEHOLDERS IN CONSERVATION OF PIG BREEDS
2.5 METHODS OF PIG BREEDS CONSERVATION
2.6 CRITERIA FOR CONSERVATION
2.6.1 POLICY
2.6.2 CONCLUSION
2.7 JUSTIFICATION/NEED FOR CONSERVING MALAWI LOCAL PIG GENETIC RESOURCES
2.7.1 GOVERNMENT POLICY ON PIG PRODUCTION
2.7.2 GOVERNMENT EFFORTS TO PROMOTE PIG FARMING IN MALAWI
2.7.3 CONSTRAINTS OF PIG PRODUCTION IN MALAWI
2.8 IMPORTANCE OF LOCAL PIGS AMONG SMALLHOLDER FARMERS
2.8.1 CHEAP SOURCE OF PROTEIN(FOOD)IN ALMOST ALL PARTS OF MALAWI
2.8.2 DISEASE AND PARASITE RESISTANCE
2.8.3 RESEARCH PURPOSE
Chapter 3 Production and Development In Vitro of Porcine Embryos derived from ICSI
3.1 INTRODUCTION
3.2 MATERIALS
3.2.1 EQUIPMENT
3.2.2 REAGENTS
3.3 METHODS
3.3.1 OOCYTE COLLECTION AND IN VITRO MATURATION
3.3.2 IN VITRO FERLIZATION
3.3.3 ICSI
3.3.4 EMBRYO CULTURE
3.3.5 ISOLATION AND IN VITRO CULTURE OF NUCLEAR DONOR CELLS
3.3.6 ENUCLEATION OF RECIPIENT OOCYTES
3.3.7 NUCLEI TRANSFER PROCEDURES
3.3.8 REAL TIME-RT-PCR
3.4 EXPERIMENTAL DESIGN
3.5 STATISTICAL ANALYSIS
3.6 RESULTS
3.6.1 Maturation in vitro of porcine oocytes
3.6.2 Effect of 50 nM TSA on developmental competence of porcine ICSI embryos
3.6.3 Detection of Aurora-A mRNA expression during the first mitosis in early stage pig embryos
3.7 DISCUSSION
CONCLUSION
References
DEDICATION
ACKNOWLEDGEMENT
本文编号：3378001
【文章来源】：南京农业大学江苏省 211工程院校 教育部直属院校
【文章页数】：94 页
【学位级别】：硕士
【文章目录】：
摘要
ABSTRACT
Part 1:Literature Review
Chapter 1:GENERAL SITUATION ON EMBRYO PRODUCTION IN FARM ANIMALS
1.1 SUCCESS OF EMBRYO PRODUCTION BY ICSI METHOD IN PIG AND OTHER FARM ANIMALS
1.2 ICSI PROCESS AND ELECTRIC ACTIVATION OF OOCYTES
1.3 THE STATUS OF IN VITRO FERTILIZATION TECHNIQUE IN EMBRYO PRODUCTION
1.4 THE PROSPECTS OF SOMATIC CELL NUCLEAR TRANSFER
1.4.1 SELECTION OF DONOR CELLS
1.4.2 ENUCLEATION OF RECIPIENT CELLS AND NUCLEAR TRANSFER
1.4.3 ACTIVATION
1.4.4 EMBRYO CULTURE
1.4.5 REMODELLING AND REPROGRAMMING
Part 2:EXPERIMENTAL STUDY
Chapter 2:MALAWI SITUATION ON PIG AND OTHER FARM ANIMAL GENETIC RESOURCESCONSERVATION
2.1 INTRODUCTION
2.1.1 PREVIOUS AND ONGOING EFFORTS BY OTHER SCIENTISTS IN MALAWI
2.1.2 CROSSBREEDING PROGRAMME
2.1.3 PIGS
2.1.4 CATTLE
2.1.5 SMALL RUMINANTS
2.1.6 CHICKENS
2.2 SELECTION
2.2.1 CATTLE
2.2.2 SMALL RUMINANTS
2.2.3 POULTRY
2.2.4 PIGS
2.3 CURRENT NATIONAL STRATEGIES FOR CONSERVATION,IMPRVEMENT AND UTILIZATION OF FARM ANIMAL GENETIC RESOURCES(AnGRs)
2.3.1 BREED SURVEY AND FIELD GUIDES
2.3.2 COMMUNITY BASED LIVESTOCK ACTIVITIES
2.3.3 PROPOSALS
2.3.4 NATIONAL BIODIVERSITY STRATEGIES ACTION PLAN
2.3.5 CONSTRAITS IN GENETIC IMPROVEMENT PROGRAMMES
2.3.6 MALAWI LOCAL PIG BREED
2.3.7 PIG NUMBERS AND OWNERSHIP PATTERN
2.4 THE VALUE OF LOCAL PIGS AMONG SMALLHOLDER FARMERS IN MALAWI
2.4.1 POVERTY ALLEVIATION PERSPECTIVE
2.4.2 AS SOURCE OF FOOD
2.4.3 TOLERANCE TO DISEASES AND PARASITES
2.4.4 DISASTER PREPAREDNESS
2.4.5 RESEARCH NEEDS
2.4.6 STRATEGIES TO CONSERVE LOCAL PIGS
2.4.7 THREATS TO INDIGENOUS PIGS
2.4.8 STAKEHOLDERS IN CONSERVATION OF PIG BREEDS
2.5 METHODS OF PIG BREEDS CONSERVATION
2.6 CRITERIA FOR CONSERVATION
2.6.1 POLICY
2.6.2 CONCLUSION
2.7 JUSTIFICATION/NEED FOR CONSERVING MALAWI LOCAL PIG GENETIC RESOURCES
2.7.1 GOVERNMENT POLICY ON PIG PRODUCTION
2.7.2 GOVERNMENT EFFORTS TO PROMOTE PIG FARMING IN MALAWI
2.7.3 CONSTRAINTS OF PIG PRODUCTION IN MALAWI
2.8 IMPORTANCE OF LOCAL PIGS AMONG SMALLHOLDER FARMERS
2.8.1 CHEAP SOURCE OF PROTEIN(FOOD)IN ALMOST ALL PARTS OF MALAWI
2.8.2 DISEASE AND PARASITE RESISTANCE
2.8.3 RESEARCH PURPOSE
Chapter 3 Production and Development In Vitro of Porcine Embryos derived from ICSI
3.1 INTRODUCTION
3.2 MATERIALS
3.2.1 EQUIPMENT
3.2.2 REAGENTS
3.3 METHODS
3.3.1 OOCYTE COLLECTION AND IN VITRO MATURATION
3.3.2 IN VITRO FERLIZATION
3.3.3 ICSI
3.3.4 EMBRYO CULTURE
3.3.5 ISOLATION AND IN VITRO CULTURE OF NUCLEAR DONOR CELLS
3.3.6 ENUCLEATION OF RECIPIENT OOCYTES
3.3.7 NUCLEI TRANSFER PROCEDURES
3.3.8 REAL TIME-RT-PCR
3.4 EXPERIMENTAL DESIGN
3.5 STATISTICAL ANALYSIS
3.6 RESULTS
3.6.1 Maturation in vitro of porcine oocytes
3.6.2 Effect of 50 nM TSA on developmental competence of porcine ICSI embryos
3.6.3 Detection of Aurora-A mRNA expression during the first mitosis in early stage pig embryos
3.7 DISCUSSION
CONCLUSION
References
DEDICATION
ACKNOWLEDGEMENT
本文编号：3378001
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