东乡绿壳蛋鸡蛋壳绿色程度相关基因的鉴定
发布时间：2021-12-18 08:46
在鸡遗传研究和绿壳蛋鸡产业中,尤其是在鸡绿壳致因突变发现后,绿壳性状是一个有趣的研究对象。在中国,已经选育出东乡绿壳蛋鸡,且该群体中绿壳蛋的致因基因SLCO1B3的显性纯合子已经被固定。尽管被固定的绿壳蛋鸡都产绿壳蛋,但蛋壳的绿色程度却深浅不一。在本研究中我们对蛋壳颜色相关的色素胆绿素与原卟啉进行了基本分析,以此获得表型性状:胆绿素含量（QB）,原卟啉含量（QP）及总色素含量（QT）,然后我们测定了与血红素通路相关的三个潜在调控基因（SLCO1B3,H01,BLVRA）的mRNA表达水平,以评估这些候选基因与蛋壳颜色强度性状的相关性。我们的研究结果显示,这些基因与该性状没有显著的关联。在前期工作中,通过全基因组关联分析,我们鉴定了一些与蛋壳颜色深浅有关的18个SNP位点,为了验证全基因组关联分析的结果,我们在两个独立的中国东乡绿壳蛋鸡群体中对鉴定到的18个SNP进行了进一步的验证工作,这18个SNP均匀分布在GGA21与蛋壳色素显著相关的区域内。验证群体包括来自于用于GWAS研究群体产生的第二个世代,包含146个个体（N146）,和另外一个来自江西东乡绿壳蛋鸡保种场的独立群体,包含28...
【文章来源】：中国农业大学北京市 211工程院校 985工程院校 教育部直属院校
【文章页数】：126 页
【学位级别】：博士
【文章目录】：
摘要
Abstract
Abbreviations
Chapter 1:Literature Review
1.1 Poultry production in China
1.2 Indigenous Blue-shelled chicken breeds in China
1.3 Egg consumption in the world and China
1.4 The color of eggshell and consumer preferences
1.5 Blue eggshell pigmentation
1.6 Genes associated with blue eggshell pigmentation
1.7 SLCO1B3 gene and EAV-HP insertion in blue-shelled chickens
1.8 Genome-wide association and the follow-up replication studies
1.9 Overview of Transcriptome-RNA-Seq studies
1.10 Introduction to RNA-Seq Technology
1.11 Application of RNA-Seq in livestock
1.12 Aim and significance of the study
1.13 Schematic representation of the workflow
CHAPTER 2:Analysis On Eggshell Pigments and Detection of mRNA Expression Levelsfor Some Related Major Genes
2.1 Introduction
2.2 Materials and methods
2.3 Results
2.4 Discussion
CHAPTER 3:A Post-GWAS Replication Studies Revealed A Promising Genomic Regionand Candidate Genes for Chicken Eggshell Blueness
3.1 Introduction
3.2 Materials and Methods
3.3 Results
3.4 Discussion
CHAPTER 4 Transcriptomic Profile of Eggshell Gland from Dark and Light Blue Eggshell
4.1 Introduction
4.2 Materials and methods
4.3 Results
4.4 Discussion
CHAPTER 5: Conclusions
5.1 Standard pigment and expression analysis
5.2 Replication association study
5.3 Transcriptome analysis
References
Acknowledgments
Appendices
【参考文献】：
期刊论文
[1]利用转录组测序筛选与鸭青壳性状形成相关的基因[J]. 陈黎,黄学涛,田勇,陶争荣,卢立志. 农业生物技术学报. 2016(07)
[2]鸡血红素加氧酶-1（HO-1）和胆绿素还原酶A（BLVRA）基因表达量对蛋壳颜色的影响[J]. 王晓庆,徐廷生,雷雪芹,高灵照,肖赞奇. 中国农学通报. 2014(35)
本文编号：3542048
【文章来源】：中国农业大学北京市 211工程院校 985工程院校 教育部直属院校
【文章页数】：126 页
【学位级别】：博士
【文章目录】：
摘要
Abstract
Abbreviations
Chapter 1:Literature Review
1.1 Poultry production in China
1.2 Indigenous Blue-shelled chicken breeds in China
1.3 Egg consumption in the world and China
1.4 The color of eggshell and consumer preferences
1.5 Blue eggshell pigmentation
1.6 Genes associated with blue eggshell pigmentation
1.7 SLCO1B3 gene and EAV-HP insertion in blue-shelled chickens
1.8 Genome-wide association and the follow-up replication studies
1.9 Overview of Transcriptome-RNA-Seq studies
1.10 Introduction to RNA-Seq Technology
1.11 Application of RNA-Seq in livestock
1.12 Aim and significance of the study
1.13 Schematic representation of the workflow
CHAPTER 2:Analysis On Eggshell Pigments and Detection of mRNA Expression Levelsfor Some Related Major Genes
2.1 Introduction
2.2 Materials and methods
2.3 Results
2.4 Discussion
CHAPTER 3:A Post-GWAS Replication Studies Revealed A Promising Genomic Regionand Candidate Genes for Chicken Eggshell Blueness
3.1 Introduction
3.2 Materials and Methods
3.3 Results
3.4 Discussion
CHAPTER 4 Transcriptomic Profile of Eggshell Gland from Dark and Light Blue Eggshell
4.1 Introduction
4.2 Materials and methods
4.3 Results
4.4 Discussion
CHAPTER 5: Conclusions
5.1 Standard pigment and expression analysis
5.2 Replication association study
5.3 Transcriptome analysis
References
Acknowledgments
Appendices
【参考文献】：
期刊论文
[1]利用转录组测序筛选与鸭青壳性状形成相关的基因[J]. 陈黎,黄学涛,田勇,陶争荣,卢立志. 农业生物技术学报. 2016(07)
[2]鸡血红素加氧酶-1（HO-1）和胆绿素还原酶A（BLVRA）基因表达量对蛋壳颜色的影响[J]. 王晓庆,徐廷生,雷雪芹,高灵照,肖赞奇. 中国农学通报. 2014(35)
本文编号：3542048
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