六种棘头虫的分子鉴定和线粒体基因组研究

发布时间：2023-04-03 04:26

　　分类学和系统学是研究生物多样性及其规律的重要工具。棘头虫是专性寄生于脊椎动物消化道内的一类重要动物寄生虫类群。目前基于比较形态学建立的棘头虫的分类标准和分类系统仍存在很多争议。核糖体DNA(rDNA)及线粒体DNA(mtDNA)序列被证明可为棘头虫的物种鉴定及系统发育分析提供有效的遗传标记。然而,目前关于棘头虫线粒体基因组的分子数据仍然很有限。迄今为止,只有17种棘头虫的线粒体基因组的完整序列被解码。因此,需要解析更多的棘头虫的线粒体基因组序列,从而为更好的解决棘头动物门的系统分类学问题提供分子数据。本研究首次测序注释了6种棘头虫的线粒体基因组全序列,包括Sphaerirostris picae(喜鹊球吻棘头虫)、S.lanceoides(似矛球吻棘头虫)、Centrorhynchus milvus(鸢中吻棘头虫)、C.clitorideus(核中吻棘头虫)、Cavisoma magnum(大腔体棘头虫)和Neoechinorhynchus sp.(新棘吻属未定种)。这6种棘头虫分别采集于巴基斯坦和中国的鱼类及鸟类体内。采用通用引物或自行设计的引物,对这6种棘头虫进行PCR扩增,获得其...

【文章页数】：111 页

【学位级别】：博士

【文章目录】：
摘要
Abstract
LIST OF ABBREVIATIONS
CHAPTER 1 INTRODUCTION
    1.1 Backgrounds
        1.1.1 Use of nuclear rDNA for identification and systematics of acanthocephalans
    1.2 Mitochondrial genome of acanthocephalans;structure and genes organization
        1.2.1 Genetic code,nucleotide composition,gene order and length of genes
        1.2.2 Transfer and ribosomal RNA genes
        1.2.3 Non-coding regions
        1.2.4 Gene overlapping
        1.2.5 Significance of mt genome in acanthocephalans taxonomy and phylogeny
    1.3 Conclusion
    1.4 Purpose and significance of the study
CHAPTER 2 Characterization of the complete mitochondrial genome of Sphaerirostris picae(Rudolphi,1819)and nearly complete mitogenome of Sphaerirostris lanceoides(Petrochenko,1949) (Acanthocephala:Centrorhynchidae)and its phylogenetic implication
    2.1 Summary
    2.2 Introduction
    2.3 Materials and methods
        2.3.1 Parasites and DNA extraction
        2.3.2 The acquisition of S.picae ITS rDNA
        2.3.3 PCR amplification and sequencing of S.picae and S.lanceoides mt genomes
        2.3.4 Phylogenetic analysis
    2.4 Results and discussion
        2.4.1 Comparison of ITS rDNA sequences of S.picae with other species in Centrorhynchidae
        2.4.2 General characteristics of S.picae and S.lanceoides mt genomes
        2.4.3 Comparison of S.picae mt genome sequences with other centrorhynchid species
        2.4.4 Phylogenetic analysis
    2.5 Conclusions
CHAPTER 3 Characterization of the complete mitogenome of Centrorhynchus milvus(Ward,1956)and Centrorhynchus Clitorideus(Meyer,1931) (Palaeacanthocephala:Centrorhynchidae),and its phylogenetic implications
    3.1 Summary
    3.2 Introduction
    3.3 Materials and methods
        3.3.1 Parasite collection
        3.3.2 Molecular procedures
        3.3.3 Phylogenetic analysis
    3.4 Results and discussion
        3.4.1 Genome organization and base composition
        3.4.2 Protein-coding genes and codon usage
        3.4.3 Transfer and ribosomal RNA genes
        3.4.4 Non-coding region
        3.4.5 Phylogenetic analysis
    3.5 Conclusion
CHAPTER 4 Characterization of the complete mitochondrial genome of Cavisoma magnum(Southwell,1927) (Acanthocephala:Palaeacanthocephala),first representative of the family Cavisomidae,and its phylogenetic implications
    4.1 Summary
    4.2 Introduction
    4.3 Materials and methods
        4.3.1 Parasites collection
        4.3.2 Molecular procedures
        4.3.3 Phylogenetic analysis
    4.4 Results and discussion
        4.4.1 Gene content and organization
        4.4.2 Protein-coding genes and codon usage
        4.4.3 Ribosomal and transfer RNA genes
        4.4.4 Non-coding regions
        4.4.5 Gene order
        4.4.6 Phylogenetic analysis
    4.5 Conclusion
CHAPTER 5 Characterization of the complete mitogenome of Neoechinorhynchus spp. (Eoacanthocephala:Neoechinorhyn chidae),the smallest mitochondrial genome in Acanthocephala,and its phylogenetic implications
    5.1 Summary
    5.2 Introduction
    5.3 Materials and methods
        5.3.1 Parasites Collection
        5.3.2 Molecular procedures
        5.3.3 Phylogenetic analysis
    5.4 Results and discussion
        5.4.1 Protein-coding genes and codon usage
        5.4.2 Ribosomal and transfer RNA genes
        5.4.3 Non-coding regions
        5.4.4 Phylogeny
    5.5 Conclusions
References
Acknowledgement
Resume



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