高氧诱导肺损伤新生小鼠肺组织中长链非编码RNANANCI的表达及对NKX2.1的调控作用

发布时间：2018-01-26 01:00

本文关键词： 高氧肺损伤长链非编码RNA NANCI NKX. 小鼠　出处：《中国当代儿科杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的探讨长链非编码RNA NANCI在高氧诱导肺损伤新生小鼠肺组织中的表达变化及对NKX2.1的调控作用。方法采用随机分组法将48只C57BL/6J新生小鼠随机分为空气组和高氧组,每组24只,各组再随机分为生后7 d组、14 d组、21 d组,每组8只。空气组置于室内环境(Fi O2=21%)中饲养,高氧组置于高氧箱(保持氧浓度95%)中饲养,在各时间点分别处死两组动物后收集肺组织标本。采用苏木精-伊红染色法观察小鼠肺组织病理变化;采用RT-q PCR及Western blot技术分别检测NANCI、NKX2.1的m RNA和蛋白表达水平。结果空气组新生小鼠肺组织NANCI和NKX2.1的m RNA表达水平7 d最高(P0.05),14 d与21 d呈同水平表达。与空气组比较,高氧组肺组织肺泡化程度降低,辐射状肺泡计数(RAC)减少(P0.05),且高氧组RAC值随高氧处理时间延长逐渐降低(P0.05)。各时间点高氧组NANCI m RNA、NKX2.1 m RNA及其蛋白表达水平均低于空气组(P0.05),且随高氧处理时间延长逐渐降低(P0.05)。NKX2.1与NANCI表达呈正相关(r=0.585,P=0.003);两者与高氧组RAC水平均呈正相关(分别r=0.655、0.541,P0.05)。结论 NANCI可能主要参与未成熟肺组织发育;肺组织损伤程度随高氧暴露时间的延长逐渐加重,且NANCI及NKX2.1水平与肺损伤程度相关,提示NANCI/NKX2.1靶基因信号通路可能参与新生小鼠高氧肺损伤过程。
[Abstract]:Objective to investigate the long chain noncoding RNA. Changes of NANCI expression in lung tissue of neonatal mice with hyperoxia-induced lung injury and its regulatory effect on NKX2.1. Methods 48 C57BL / 6J newborn mice were randomly divided into empty by random grouping. Gas group and hyperoxia group. 24 rats in each group were randomly divided into two groups: the 7th day postnatal group, 14 d group, 21 d group, 8 rats in each group, and the air group was fed in indoor environment. The rats in the hyperoxia group were fed in the hyperoxia box (keeping oxygen concentration 95). The lung tissue samples were collected after the animals were killed at each time point. The pathological changes of lung tissue were observed by hematoxylin-eosin staining method. NANCI was detected by RT-q PCR and Western blot. Results the expression level of m RNA in lung tissue of newborn mice in air group was highest at 7 days (P 0.05). Compared with the air group, the alveolar degeneration of lung tissue in the hyperoxia group decreased, and the radiative alveolar count decreased (P 0.05). The RAC value of hyperoxia group decreased gradually with the prolongation of hyperoxia treatment time, and NANCI m RNA of hyperoxia group decreased gradually at each time point. The expression level of NKX2.1 m RNA and its protein was lower than that of air group (P 0.05). With the prolongation of hyperoxia treatment time, the expression of P0.05N. NKX2.1 was positively correlated with the expression of NANCI. There was a positive correlation between NANCI and RAC level in hyperoxia group (r = 0.655-0.541P 0.050.Conclusion NANCI may be involved in the development of immature lung tissue. The degree of lung injury increased with the prolongation of hyperoxia exposure time, and the levels of NANCI and NKX2.1 were correlated with the severity of lung injury. These results suggest that NANCI/NKX2.1 target gene signaling pathway may be involved in the process of hyperoxia lung injury in newborn mice.
【作者单位】：南京医科大学附属淮安第一医院新生儿科;
【基金】：江苏省临床医学专项(BL2014063) 淮安市科技攻关项目(HAS2014010)
【分类号】：R722.1
【正文快照】： 支气管肺发育不良(bronchopulmonary dysplasia,BPD)是发生于早产儿长期应用高浓度氧气和辅助机械通气后的一种慢性肺疾病(chronic lungdisease,CLD),是由生后感染、气压伤和容量伤导致的纤维化以及氧毒性等多因素诱发形成[1],其发病率随胎龄和出生体重的减少而增高[2],其发生

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