2014-2015年广州759例儿童肠道病毒感染病原谱调查

发布时间：2018-01-26 11:58

本文关键词： 肠道病毒属聚合酶链反应手足口病　出处：《中华疾病控制杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的调查2014-2015年我院759例儿童感染肠道病毒血清型的分布,了解广州地区肠道病毒(enterovirus,EV)病原谱。方法采集手足口病等疑似肠道病毒感染患儿肛拭子标本,采用荧光逆转录多聚酶链反应法(reverse transcriptase polymerase chain reaction,RT-PCR)检测通用型、肠道病毒71型(enterovirus 71,EV71)柯萨奇病毒A组16型(coxsackie virus A group 16,CA16)核酸,巢式PCR法扩增VP1基因测序分型。结果 1 060例疑似感染者检出759例EV阳性(检出率71.6%);两年均检出11种血清型,2014年CA16、EV71、CA4、CA10和CA6位列前五位,CA16和EV71占77.1%;2015年CA6、EV71、CA10、CA16和CA2列前五位,CA6占51.7%;性别比为1.8~5.3;感染以3岁儿童为主(60.1%,456/759);全年均可检出,4~7月达高峰,5月CA16、EV71、CA10和CA2达高峰,7月CA6达高峰。由于病原谱不同,2015年病情普遍较2014年轻,两年均无死亡病例。EV71感染重症病例比率最高(12.7%),CA16、CA10、CA4和CA6偶见重症病例。结论广州主要流行十余种肠道病毒,两年病原谱不同,大部分感染引起疾病较轻。
[Abstract]:Objective to investigate the distribution of enterovirus serotypes in 759 children infected with enterovirus in our hospital in 2014-2015. Methods the anal swabs of children with suspected enterovirus infection such as hand, foot and mouth disease were collected. Reverse transcriptase polymerase chain reaction was detected by fluorescence reverse transcriptase polymerase chain reaction (FRT). RT-PCR was used to detect enterovirus 71. EV71) Coxsackie virus A group 16CA16) nucleic acid. VP1 genotyping was amplified by nested PCR. Results 759 EV-positive cases were detected in 1 060 suspected infected patients (the detection rate was 71.6%). In 2014, CA16 EV71, CA4, CA10 and CA6 ranked the top five, CA16 and EV71 accounted for 77.1%. In 2015, CA6 EV71, CA10, CA16, and CA2, the top five, accounted for 51.7 percent of the total. The sex ratio was 1.8 卤5.3; The infection was mainly in children aged 3 years, 60.1% and 456 / 759%; During the whole year, the peak was detected from 4 to July, CA16 EV71, CA10 and CA2 reached its peak on May, and CA6 reached its peak on July, due to different pathogenetic profiles. In 2015, the disease was generally younger than that in 2014, and there were no death cases in two years. The rate of severe cases infected with EV71 was the highest (12.7%). CA4 and CA6 are rare severe cases. Conclusion there are more than ten kinds of enterovirus in Guangzhou, the pathogeny spectrum is different in two years, and most of the infections cause mild diseases.
【作者单位】：南方医科大学珠江医院检验医学部;广东省突发传染病病原微生物重点实验室;
【基金】：“十二五”国家科技重大专项(2014ZX 10004005)
【分类号】：R440;R725.1
【正文快照】： (Chin J Dis Control Prev 2017,21(6):567-571)2广东省突发传染病病原微生物重点实验室,广东广州510282儿童肠道病毒(enterovius,EV)感染非常常见,表现为手足口病(hand-foot-mouth disease,HFMD)、疱疹性咽峡炎、呼吸道感染、神经系统感染等[1]。手足口病最为普遍,2008-2015

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