EV71感染对手足口病患儿DCs成熟、凋亡及MAPK信号通路和炎性细胞因子表达的影响

发布时间：2018-02-01 13:14

本文关键词： 肠道病毒型树突状细胞 MAPK信号通路炎性细胞因子　出处：《山东医药》2017年09期 　论文类型：期刊论文

【摘要】：目的探究肠道病毒71型(EV71)感染对手足口病患儿树突状细胞(DCs)成熟、凋亡及MAPK信号通路和炎性细胞因子表达的影响。方法选择手足口病患儿40例,根据感染程度将患儿分为轻症感染组(20例)、重症感染组(20例),另选取同期同年龄段健康儿童20例作为对照组。抽取各组DCs体外培养、诱导后检测表面标志的变化、细胞凋亡情况及MAPK信号通路活化情况,并对细胞培养上清中炎性细胞因子表达水平进行检测。结果轻度感染组、重度感染组CD80、CD83及CD86阳性率随感染程度的加重而上升,其中CD80、CD83与对照组比较差异有统计学意义(P均0.05)。对照组凋亡细胞检出率及死亡细胞检出率在三组中最高;轻度感染组凋亡细胞及死亡细胞检出率与对照组相比降低;重度感染组凋亡细胞及死亡细胞检出率最低;三组细胞凋亡率及死亡率比较差异有统计学意义(P均0.05)。轻度感染组、重度感染组p-p38 MAPK、p-JNK、p-ERK1/2蛋白表达量均随感染程度加深而增加(P均0.05)。IL-6及IL-12表达随感染程度的加重而呈上升趋势,其中IL-6变化明显,三组间比较差异有统计学意义(P均0.05),IL-12略有升高,三组间比较差异无统计学意义(P均0.05);TNF-α表达随感染程度的加重呈先升高后降低的趋势,三组间比较差异有统计学意义(P均0.05)。结论 EV71感染可促进DCs成熟并分泌细胞因子,同时抑制其凋亡,活化MAPK信号通路,上调炎性细胞因子表达。
[Abstract]:Objective to investigate the effect of enterovirus 71 (EV71) infection on dendritic cell (DC) maturation in children with hand, foot and mouth disease (HFMD). Methods 40 children with hand-foot-mouth disease were divided into two groups according to the degree of infection. 20 cases of severe infection group and 20 cases of healthy children of the same age were selected as control group. The DCs of each group was cultured in vitro and the changes of surface markers were detected after induction. Apoptosis and activation of MAPK signal pathway and expression of inflammatory cytokines in the supernatant of cell culture were detected. Results CD80 was detected in mild infection group and severe infection group. The positive rates of CD83 and CD86 increased with the severity of infection, including CD80. The difference between CD83 and control group was statistically significant (P < 0.05). The detection rate of apoptotic cells and dead cells in the control group was the highest in the three groups. The detection rate of apoptotic cells and dead cells in mild infection group was lower than that in control group. The detection rate of apoptotic cells and dead cells was the lowest in severe infection group. There were significant differences in apoptosis rate and death rate among the three groups (P < 0.05). In mild infection group, severe infection group, p-p38 MAPKN p-JNK. The expression of p-ERK1 / 2 protein increased with the degree of infection, and the expression of IL-6 and IL-12 increased with the severity of infection. The change of IL-6 was obvious, the difference between the three groups was statistically significant (P < 0.05), but there was no significant difference among the three groups (P < 0.05). The expression of TNF- 伪 increased first and then decreased with the severity of infection. Conclusion EV71 infection can promote DCs maturation, secrete cytokines, inhibit its apoptosis and activate MAPK signaling pathway. Upregulate the expression of inflammatory cytokines.
【作者单位】：盘锦职业技术学院医学分院;
【分类号】：R725.1
【正文快照】： 肠道病毒71型(EV71)是儿童手足口病的主要致病源[1],其感染人数逐年上升。EV71感染所致手足口病起病急、发展快[2],轻度感染患儿预后良好,重度感染患儿多伴随脑膜炎等并发症[3],且致残致死率高。目前针对该类手足口病尚无有效的疫苗及药物。研究显示EV71可使感染患儿体液免疫

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