呼吸道合胞病毒感染对哮喘小鼠RANTES表达的影响

发布时间：2018-03-03 16:45

本文选题：哮喘　切入点：呼吸道合胞病毒　出处：《临床儿科杂志》2012年03期 　论文类型：期刊论文

【摘要】：目的研究呼吸道合胞病毒(RSV)感染对哮喘小鼠RANTES(regulated upon activation normal T cellexpressed and secreted)生成的影响。方法将BALB/c小鼠分为对照组、哮喘组、RSV感染组和RSV感染哮喘组4组,每组各8只。先采用卵清白蛋白致敏法制作小鼠哮喘模型,随后以RSV感染小鼠,RSV感染后24 h处死小鼠。检测小鼠支气管肺泡灌洗液(BALF)中RANTES及IFN-γ的浓度,并计数炎性细胞数。结果 RSV感染组和对照组,RSV感染哮喘组和哮喘组小鼠之间,BALF中的RANTES浓度差异均有统计学意义(P均0.05);而对照组和哮喘组,RSV感染组和RSV感染哮喘组小鼠之间的RANTES浓度差异则无统计学意义(P均0.05)。各组小鼠BALF中的IFN-γ浓度差异无统计学意义。哮喘组、RSV感染组、RSV感染哮喘组小鼠BALF中的各种炎性细胞计数均较对照组升高,差异均有统计学意义(P0.05),哮喘小鼠以嗜酸性粒细胞增高为主,而RSV感染小鼠以中性粒细胞为主。结论感染RSV可使小鼠RANTES的表达增加,但哮喘未能使小鼠RANTES表达增加,提示RANTES增加可能与RSV感染有关,而与哮喘状态无关。
[Abstract]:Objective to study the effect of respiratory syncytial virus (RSV) infection on the production of RANTES(regulated upon activation normal T cellexpressed and secreded in asthmatic mice. Eight mice in each group were first sensitized with ovalbumin to make asthma model, then mice were killed 24 hours after RSV infection. The concentrations of RANTES and IFN- 纬 in bronchoalveolar lavage fluid (BALF) were measured. Results there were significant differences in RANTES concentration between RSV infected group and control group in asthmatic group and asthma group (P < 0.05), while in control group and asthma group, there was significant difference in RANTES concentration in RSV infection group and asthma group, while in control group and asthma group, there was significant difference in RANTES concentration between the two groups (P < 0.05). There was no significant difference in RANTES concentration between the two groups (P < 0.05). There was no significant difference in the concentration of IFN- 纬 in BALF between the two groups. The number of inflammatory cells in BALF of the asthmatic group was higher than that of the control group. The difference was statistically significant (P 0.05). Eosinophilic granulocytes increased mainly in asthmatic mice and neutrophil in RSV infected mice. Conclusion RSV infection can increase the expression of RANTES in mice, but asthma does not increase the expression of RANTES in mice. The results suggest that the increase of RANTES may be related to RSV infection, but not to asthma.
【作者单位】：吉林大学白求恩第一医院小儿呼吸科;
【分类号】：R725.6

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