雾化吸入一氧化氮供体硝普钠治疗新生猪ARDS的实验研究

发布时间：2018-03-06 04:01

本文选题：急性呼吸窘迫综合征　切入点：一氧化氮供体　出处：《南华大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的探讨雾化吸入(neb)一氧化氮（NO）供体硝普钠（SNP）(neb-SNP)对新生猪急性呼吸窘迫综合征（ARDS）的疗效、安全性及实验依据. 方法采用生理盐水（NS）肺泡灌洗法复制ARDS新生猪模型（n=30）。造模成功后将其随机分成5组(n=6×5)。A组（对照组）：仅气管插管，接呼吸机进行机械通气治疗；B组（NS组）：机械通气治疗，并经呼吸机管道nebNS30min；C，D和E组为实验组，机械通气治疗，并经呼吸机管道分别neb-SNP1mg/mL，5mg/mL和10mg/mL30min。对5组分别进行连续平均股动脉压(MAP)、平均肺动脉压(MPAP)及全血高铁血红蛋白(MetHb)含量监测,分别于基础状态、造模型成功时、雾化结束时和雾化结束后30min，60min，120min进行动脉血气分析和细胞因子，如肿瘤坏死因子（TNF）-α与白细胞介素（IL）-10，-12测定。结果：造模型成功时,B，C，D，E组MPAP均显著高于A组，差异有统计学意义(P＜0.01)；雾化结束时, MPAP：D和E组较B组显著下降，差异有统计学意义(P＜0.05)。在相同时点, MAP：5组比较，差异无统计学意义(P＞0.05).雾化结束时,全血MetHb含量5组比较，差异无统计学意义(P＞0.05)。C，D和E组雾化结束后30min，未发现有反跳性肺动脉高压。neb结束后不同时点，C，D，E与A，B组IL-12，IL-10，TNF-α比较，差异有统计学意义(P＜0.05)，而D与E组比较，差异无统计学意义(P＞0.05). 结论：neb-SNP可选择性降低ARDS模型肺动脉高压，同时具有调节炎症介质和抗炎因子等作用，，从而减轻ARDS症状。
[Abstract]:Objective to investigate the efficacy, safety and experimental evidence of nebulized sodium nitroprusside (SNPN) in the treatment of acute respiratory distress syndrome (ARDS) in newborn pigs. Methods the ARDS new pig model was made by alveolar lavage with normal saline (NS). After the successful establishment of the model, the model was randomly divided into 5 groups (control group: endotracheal intubation only, ventilator treated group NS: mechanical ventilation therapy). The experimental group was treated with mechanical ventilation and the neb-SNP1 mg / mL-1 mg / mL and 10 mg / mL30mins were treated by mechanical ventilation respectively. The mean femoral arterial pressure (MAPP), mean pulmonary artery pressure (MPAP) and whole blood methemoglobin (MetHb) content were monitored respectively in the five groups. Arterial blood gas and cytokines, such as tumor necrosis factor TNF- 伪 and interleukin (IL) -10 ~ (-10) -12, were determined at the end of atomization and 30 min after atomization, respectively, at the basic state, at the time of successful establishment of the model and at 60 min or 120 min after nebulization. Results: when the model was successful, the MPAP of group E was significantly higher than that of group A (P < 0.01), and at the end of atomization, MPAP:D and group E were significantly lower than those of group B (P < 0.05). At the same time, the MAP:5 group was significantly lower than that of group B (P < 0.05). At the end of nebulization, there was no significant difference in whole blood MetHb content between group A and group E (P > 0.05). There was no significant difference between group E and group E at 30 min after atomization, and there was no significant difference between group IL-12 IL-10 TNF- 伪 and group IL-12 IL-10 TNF- 伪 at different time points after neb. The difference was statistically significant (P < 0.05), but there was no significant difference between group D and group E (P > 0.05). Conclusion:% neb-SNP can selectively reduce pulmonary hypertension in ARDS model and regulate inflammatory mediators and anti-inflammatory factors so as to alleviate the symptoms of ARDS.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R722.1

【相似文献】