乌司他丁干预对脓毒症幼鼠血清肿瘤坏死因子-α、P-选择素和凝血酶抗凝血酶复合物水平的影响

发布时间：2018-03-07 01:07

本文选题：乌司他丁　切入点：脓毒症　出处：《中国当代儿科杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的探讨使用乌司他丁(UTI)作为早期干预药物对脓毒症幼鼠血清肿瘤坏死因子-α(TNF-α)、P-选择素、凝血酶抗凝血酶复合物(TAT)水平的影响及意义。方法将120只4周龄雄性大鼠随机分为正常对照组、假手术组、脓毒症组、低剂量UTI干预组(50 000 U/kg)和高剂量UTI干预组(200 000 U/kg),每组24只。采用改良的盲肠结扎穿孔法制作脓毒症大鼠模型,低剂量和高剂量UTI干预组在建模后分别尾静脉注射UTI。ELISA法测定6、12、24 h时各组大鼠血清TNF-α、P-选择素、TAT水平。结果脓毒症组大鼠血清TNF-α、TAT、P-选择素水平在6 h时即出现显著升高,血清TNF-α、TAT水平至24 h时仍呈持续升高状态(P0.05);P-选择素在12 h时达到峰值,24 h时出现回落(P0.05)。UTI干预组中,P-选择素和TAT水平变化趋势和脓毒症组相同,TNF-α水平在6 h出现明显的增高,但随时间延长逐渐降低,且变化幅度均显著小于脓毒症组(P0.05);高剂量组变化幅度显著小于低剂量组(P0.05)。结论 UTI早期干预对脓毒症幼鼠模型的凝血功能有明显的改善,能抑制TNF-α、P-选择素、TAT的生成,且高剂量组较低剂量组作用更显著。
[Abstract]:Objective to explore the effect of ulinastatin (UTI) as an early intervention drug on serum tumor necrosis factor- 伪 (TNF- 伪) and P- selectin in young rats with sepsis. Methods 120 4-week-old male rats were randomly divided into normal control group, sham operation group and sepsis group. Low dose UTI intervention group (50 000 U / kg) and high dose UTI intervention group (200 000 U / kg) were used to make sepsis rat model by modified cecal ligation and perforation method. The serum levels of TNF- 伪 -P- selectin (TNF- 伪 -TNF- 伪 -TATP-selectin) were measured in the low dose and high dose UTI intervention groups at 24 h after modeling, respectively. Results the serum TNF- 伪 -TTATP-selectin level in the septic group increased significantly at 6 h after injection of TNF- 伪 -TATP-selectin. The serum TNF- 伪 tat level continued to increase at 24 h. The level of P0.05 and P- selectin reached its peak at 12 h and reached the peak at 24 h. The change trend of serum TNF- 伪 and TNF- 伪 levels in the intervention group was significantly higher than that in the septic group at 6 h, and the level of TNF- 伪 in the intervention group was significantly higher than that in the septic group at 6 h, and the serum TNF- 伪 level in the intervention group was significantly higher than that in the septic group at 6 h. However, the changes were decreased gradually with the prolongation of time, and were significantly lower than those of sepsis group (P 0.05) and high dose group (P 0.05). Conclusion early intervention of UTI can improve the coagulation function of septic young rats. It could inhibit the production of TNF- 伪 -P- selectin tat, and the effect of high dose group was more obvious than that of low dose group.
【作者单位】：南昌大学第一附属医院儿科;
【分类号】：R720.597

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