高脂饮食对小鼠呼吸道和背根神经节瞬时感受器电位阳离子通道香草酸-1的影响

发布时间：2018-04-04 02:56

本文选题：高脂饮食　切入点：气道　出处：《中国当代儿科杂志》2017年07期

【摘要】：目的探讨高脂饮食对瞬时感受器电位阳离子通道香草酸-1(TRPV1)在小鼠呼吸系统和背根神经节(DRG)上的表达变化,并研究其对感觉神经元兴奋性的影响。方法将C57BL/6小鼠20只随机分成普通饲料组和高脂饲料组,每组10只。分别喂养相应饲料并监测体重。喂养7周后取两组小鼠肺叶、支气管组织,以及胸3~4段DRG行免疫组化染色检测TRPV1表达水平,并用膜片钳测量DRG动作电位和TRPV1电流。结果经7周饲养后,高脂饲料组平均增重(6.4±2.6 g)显著大于普通饲料组(2.3±0.5 g)(P0.001)。高脂饲料组支气管、肺泡和DRG上TRPV1的阳性表达均明显高于普通饲料组(P0.05)。高脂饲料组DRG上TRPV1电流强度和动作电位激发数目较普通饲料组均明显升高(P0.05)。结论高脂饮食引起小鼠体重明显增加,导致小鼠呼吸系统和外周感觉神经元中TRPV1呈现高表达和易激惹高兴奋性,提示TRPV1可能是导致支气管超高反应病理生理机制中的一个重要因子。
[Abstract]:Objective to investigate the effect of high fat diet on the expression of vanillic acid-1 (vanillic acid-1) in the respiratory system and dorsal root ganglion (DRG) of mice and its effect on the excitability of sensory neurons.Methods Twenty C57BL/6 mice were randomly divided into normal diet group and high fat diet group with 10 mice in each group.The corresponding feed was fed and the body weight was monitored.After feeding for 7 weeks, the expression of TRPV1 was detected by immunohistochemical staining in lung lobes, bronchi and 4 segments of thoracic DRG of mice. DRG action potential and TRPV1 current were measured with patch clamp.Results after feeding for 7 weeks, the average weight gain of the high-fat diet group was 6.4 卤2.6 g, which was significantly higher than that of the normal diet group (2.3 卤0.5 g / kg, P 0.001).The positive expression of TRPV1 in bronchi, alveoli and DRG in high fat diet group was significantly higher than that in normal diet group.The number of TRPV1 current and action potential excitation on DRG in high fat diet group was significantly higher than that in normal diet group.Conclusion High fat diet can significantly increase the body weight of mice and induce high expression of TRPV1 in respiratory system and peripheral sensory neurons of mice.It suggests that TRPV1 may be an important factor in the pathophysiological mechanism of bronchial hyperthermia.
【作者单位】：武汉大学人民医院儿科;孝感市妇幼保健院儿科;
【分类号】：R725.6

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