儿科患者鲍曼不动杆菌氨基糖苷类修饰酶基因的表达

发布时间：2018-05-04 01:20

  本文选题：鲍曼不动杆菌 + 儿科患者　； 参考：《中国抗生素杂志》2009年03期

【摘要】：目的研究从儿科肺炎患者中分离的鲍曼不动杆菌对氨基糖苷类抗生素的耐药性和常见的7种氨基糖苷类修饰酶基因特征。方法56株鲍曼不动杆菌(AB)收集自2006年分离的儿科临床肺炎患儿的深部痰培养标本,均采用VITEK-32全自动微生物鉴定仪GNI和GNS卡进行细菌鉴定和药敏试验。氨基糖苷类修饰酶基因检测采用聚合酶链式反应(PCR)方法。结果检测的56株鲍曼不动杆菌菌有8株呈多重耐药性,阳性率14.29%,8株多重耐药菌对氨基糖苷类药物阿米卡星、妥布霉素和庆大霉素均耐药,其余菌株对氨基糖苷类药物均敏感,氨基糖苷类药物的耐药率14.29%。除呋喃妥因及β-内酰胺类抗生素氨苄西林、头孢唑林和头孢曲松外其它抗生素的耐药率均在15%以下。7种氨基糖苷类修饰酶基因中检出2株aac(3)-Ⅱ(3.57%),2株aac(6')-Ⅰb(3.57%),4株aac(6')-Ⅱ(7.14%),6株ant(3″)-Ⅰ(10.71%);aac(3)-Ⅰ、ant(2″)-Ⅰ和aac(6')-Ⅰad均阴性。对3种氨基糖苷类抗生素耐药的菌株均检出了氨基糖苷类修饰酶基因。结论(1)儿科患者虽然极少应用氨基糖苷类抗生素,但由于氨基糖苷类修饰酶基因可借助于整合子、转座子和质粒等在同种和异种细菌间传播,使其对氨基糖苷类抗生素也产生了一定的耐药性。(2)鲍曼不动杆菌儿科患者分离株对阿米卡星、庆大霉素和妥布霉素的耐药与aac(3)-Ⅱ、aac(6')-Ⅰb、aac(6')-Ⅱ和ant(3″)-Ⅰ四种基因有关。不同地区细菌的修饰酶基因有很大差异,而儿童与成人患者亦有不同,因此要重视儿科患者鲍曼不动杆菌氨基糖苷类抗生素耐药性和耐药基因研究。
[Abstract]:Objective to study the antimicrobial resistance of Acinetobacter baumannii to aminoglycoside antibiotics and the characteristics of seven common aminoglycoside modified enzyme genes in pediatric patients with pneumonia. Methods 56 strains of Acinetobacter baumannii were collected from pediatric children with clinical pneumonia in 2006. The bacteria were identified by VITEK-32 GNI and GNS card. Aminoglycoside modified enzyme gene was detected by polymerase chain reaction (PCR). Results among 56 strains of Acinetobacter baumannii, 8 strains showed multidrug resistance. The positive rate was 14.29% and 8 strains were resistant to aminoglycoside drugs such as amikacin, tobramycin and gentamicin. The other strains were sensitive to aminoglycosides. The resistance rate of aminoglycosides was 14.29%. Defurantoin and 尾-lactam antibiotics ampicillin, The resistance rates of cefazolin and ceftriaxone to other antibiotics were below 15%. Two of the 7 aminoglycoside modified enzyme genes were found to be negative in two strains of aacantoin (3. 57)-1. 3. 57 and 4 strains of aacanthus 6-2. 7. 14. 14. 6 strains of ant(3 "- 鈪，

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