肺炎支原体肺炎与肺表面活性物质相关蛋白A的相关性研究

发布时间：2018-05-10 21:11

  本文选题：肺表面活性物质 + 肺炎支原体　； 参考：《南京医科大学学报(自然科学版)》2017年06期

【摘要】：目的:探讨肺表面活性物质相关蛋白A(surfactant protein-A,SP-A)在肺炎支原体肺炎(Mycoplasma pneumoniae pneumonia,MPP)中的作用及临床意义。方法:收集2014年1月1日—2015年12月31日在南京医科大学附属儿童医院住院的肺炎患儿共51例:其中MPP患儿41例,分别为MPP组15例,MPP伴肺不张组26例,同时设立同期入院非支原体感染的普通肺炎10例为对照组,采用ELISA法检测各组患儿血清和肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中SP-A含量,逆转录聚合酶链反应方法(RT-PCR)检测血清SP-A m RNA相对表达情况。结果:(1)MPP组、MPP伴肺不张组血清、BALF中SP-A蛋白水平均低于对照组,差异均有统计学意义(P0.05),但MPP组、MPP伴肺不张组之间差异无统计学意义(P0.05)。(2)MPP组及MPP伴肺不张组血清SP-A含量及m RNA水平与BALF中SP-A蛋白含量均呈正相关(r=0.42,P=0.01;r=0.41,P=0.04)。(3)MPP伴肺不张组血清、BALF中SP-A含量及血清SP-A m RNA水平与肺复张时间无明显相关性(P值均0.05)。结论:MPP患儿血清、BALF中SP-A蛋白含量明显下降,MPP中可能存在肺泡Ⅱ型细胞损伤,SP-A的替代治疗或促进SP-A生成的治疗对MPP的恢复将有一定意义。
[Abstract]:Objective: to investigate the role of pulmonary surfactant associated protein (A(surfactant protein-An SP-A) in Mycoplasma pneumoniae pneumonia (Mycoplasma pneumoniae) and its clinical significance. Methods: from January 1, 2014 to December 31, 2015, 51 children with pneumonia were enrolled in the affiliated Children's Hospital of Nanjing Medical University, including 41 patients with MPP, including 15 patients with MPP and 26 patients with atelectasis. At the same time, 10 cases of common pneumonia with non-mycoplasma infection were used as the control group. The levels of SP-A in serum and bronchoalveolar lavage fluidform (BALF) of children in each group were detected by ELISA method. Reverse transcriptase polymerase chain reaction (RT PCR) was used to detect the relative expression of SP-A m RNA in serum. Results the level of SP-A protein in serum of MPP group with atelectasis was lower than that of control group. The difference was statistically significant (P 0.05), but there was no significant difference between MPP group and MPP with atelectasis group. There was a positive correlation between the level of serum SP-A and m RNA and the content of SP-A protein in BALF. There was no significant correlation between the level of SP-A and the level of serum SP-A m RNA and lung extension time (P = 0.05). Conclusion the level of SP-A protein in the serum of children with BALF decreased significantly. There may be replacement therapy of SP-A for alveolar type 鈪，

本文编号：1870923