表没食子儿茶素没食子酸酯对肠缺血再灌注损伤的保护作用研究

发布时间：2018-06-06 09:13

本文选题：表没食子儿茶素没食子酸酯 + 肠缺血再灌注损伤　；参考：《重庆医学》2017年20期

【摘要】：目的探讨表没食子儿茶素没食子酸酯(EGCG)对大鼠肠缺血再灌注损伤的影响及其作用机制。方法 40只SD大鼠均分为4组:假手术组(Sham组)、肠道缺血再灌注损伤组(IRI组)、EGCG预处理组(EGCG组)和Wnt激动剂HLY78组(Wnt-Ag组)。IRI、EGCG和Wnt-Ag组无损伤血管夹夹闭肠系膜上动脉(SMA)45min构建肠缺血再灌注损伤模型,Sham组只做假手术处理。EGCG组在缺血前45 min腹腔注射EGCG(50 mg/kg),Wnt-Ag组在缺血前45 min腹腔注射EGCG(50mg/kg)+Wnt-Ag(5mg/kg),Sham组和IRI组腹腔注射等量生理盐水。再灌注4h后HE染色观察肠组织病理形态;免疫组织化学检测细胞凋亡;RT-PCR和ELISA检测肠道和血清肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、白细胞介素1(IL-1)表达,Western blot法检测肠组织Wnt、β-catenin、p53、Bax和BCL-2表达。结果与Sham组相比,IRI组IL-6、IL-1、TNF-α、Wnt、β-catenin、p53、Bax、细胞凋亡和肠道病理改变明显增加,而BCL-2表达明显减少。与IRI组相比,EGCG组IL-6、IL-1、TNF-α、Wnt、β-catenin、p53、Bax、细胞凋亡和肠道病理改变明显减少,而BCL-2表达明显增加。与EGCG组相比,Wnt-Ag组IL-6、IL-1、TNF-α、Wnt、β-catenin、p53、Bax、细胞凋亡和肠道病理改变明显增加,而BCL-2表达明显减少。结论 EGCG可通过抑制炎症和凋亡而减轻肠缺血再灌注损伤,这种保护作用可能是通过抑制Wnt/β-catenin信号途径介导。
[Abstract]:Objective to investigate the effects of epigallocatechin gallate (EGCG) on intestinal ischemia-reperfusion injury in rats and its mechanism. Methods Forty SD rats were divided into four groups: sham-operated group (Sham group), intestinal ischemia-reperfusion injury group (IRI group) and Wnt agonist HLY78 group (HLY78 group). The model of intestinal ischemia-reperfusion injury in Sham group was treated with sham-operation only. EGCG(50 mg / kg Wnt-Ag group was injected intraperitoneally with EGCG(50 mg / kg Wnt-Ag group at 45 min before ischemia. Wnt-Ag5 mg / kg + Sham group and IRI group were intraperitoneally injected with the same amount of normal saline. The histopathology of intestine was observed by HE staining for 4 h after reperfusion, and the expressions of Wnt, 尾 -catenin p53 Bax and BCL-2 in intestinal tissue were detected by immunohistochemistry, RT-PCR and ELISA were used to detect the expression of TNF- 伪, IL-6, IL-6 and IL-1in intestinal tissue. Results compared with Sham group, IL-6 and IL-1TNF- 伪 TNF- 伪 Wnt, 尾 -catenin p53 Bax. apoptosis and intestinal pathological changes were significantly increased, while the expression of BCL-2 was significantly decreased. Compared with IRI group, IL-6 and IL-1TNF- 伪 Wnt, 尾 -catenin p53 Bax. apoptosis and intestinal pathological changes were significantly decreased, while the expression of BCL-2 was significantly increased in EGCG group. Compared with EGCG group, the expression of IL-6 and IL-1TNF- 伪 in Wnt-Ag group was significantly higher than that in Wnt-Ag group, and the expression of TNF- 伪, 尾 -cateninin p53, Bax. apoptosis and intestinal pathological changes were significantly increased, while the expression of BCL-2 was significantly decreased in Wnt-Ag group. Conclusion EGCG can attenuate intestinal ischemia-reperfusion injury by inhibiting inflammation and apoptosis, which may be mediated by inhibiting Wnt/ 尾 -catenin signaling pathway.
【作者单位】：四川大学华西第四医院重症监护室;中航工业363医院放疗科;
【分类号】：R574

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