2014-2015年苏州市5岁以下儿童病毒性腹泻病原学分析

发布时间：2018-06-10 15:22

本文选题：病毒性腹泻 + 病原学　；参考：《现代预防医学》2017年05期

【摘要】：目的了解苏州市5岁以下儿童病毒性腹泻病原体的分布情况,为病毒性腹泻的防治提供科学依据。方法收集2014年1月至2015年12月苏州大学附属儿童医院因腹泻住院的5岁以下儿童的粪便标本,采用酶联免疫吸附试验(ELISA)检测轮状病毒(HRV),用聚合酶链反应(PCR)和逆转录聚合酶链反应(RT-PCR)检测杯状病毒(Hu CV)、肠道腺病毒(EAd V)及星状病毒(Ast V)。结果 737份样本中,HRV、Hu CV,EAd V、Ast V阳性率分别为28.09%、17.23%、3.12%、1.63%,其中混合感染36份。男、女患儿病毒检出率差异无统计学意义(=0.211,P=0.646);12~18月龄患儿病毒检出率最高(62.11%),其次为18~24月龄(60.66%),不同年龄组病毒检出率差异有统计学意义(=46.764,P0.001);病毒检出高峰集中在10月至次年1月,不同月份病毒检出率差异有统计学意义(=159.291,P0.001);207份HRV阳性样本中病毒毒株G分型以G9型为主,占86.47%,P分型以P8型为主,占93.24%。结论HRV和Hu CV是引起苏州市5岁以下儿童病毒性腹泻的主要病原体;病毒检出高峰集中在秋冬季,易感人群以2岁以下的儿童为主;HRV主要血清型为G9型、主要基因型为P8型。
[Abstract]:Objective to investigate the distribution of viral diarrhea pathogens in children under 5 years of age in Suzhou, and to provide scientific basis for the prevention and treatment of viral diarrhea. Methods from January 2014 to December 2015, fecal specimens of children under 5 years old hospitalized in the Children's Hospital affiliated to Suzhou University for diarrhea were collected. The rotavirus HRV was detected by ELISA.According to polymerase chain reaction (PCR) and reverse transcriptase polymerase chain reaction (RT-PCR), the adenovirus (Hu CVV), intestinal adenovirus (EAd V) and stellate virus (Ast VG) were detected by polymerase chain reaction (PCR) and reverse transcriptase polymerase chain reaction (RT-PCR). Results the positive rate of HRV Hu CVEAd Ast V in 737 samples was 28.09 and 17.23 and 3.123.121.63 respectively, 36 of which were mixed infection. There was no significant difference in the detection rate of virus between male and female children. The highest detection rate of virus was 0.211P0.646 / 126-month old, followed by 1824 months old, 60.660.The difference of virus detection rate between different age groups was statistically significant, and the peak of virus detection was from October to January of the following year. There were significant differences in the detection rate of virus in different months. Among 207 HRV positive samples, G9 type was the main type, and P8 type was the main type and 93.2445% in P type. Conclusion HRV and Hu CV are the main pathogens of viral diarrhea in children under 5 years of age in Suzhou, the peak of virus detection is in autumn and winter. The main serotype of HRV in susceptible population is G9, and the main genotype is P8.
【作者单位】：苏州市疾病预防控制中心;
【基金】：苏州市医学重点学科(编号:Szxk201516)
【分类号】：R725.1

【相似文献】