低出生体重新生儿血清蛋白组学分析

发布时间：2018-06-17 15:14

本文选题：低出生体重儿 + 蛋白质组学　；参考：《泸州医学院》2012年硕士论文

【摘要】：目的：低出生体重新生儿（Low birth weight infant, LBW）是指出生1小时内体重不足2500g者。一般是早产儿或小于胎龄儿，小于龄儿（small for gestational age, SGA）又称胎儿宫内生长迟缓（intrauterinegrowth retardation, IUGR）。本实验应用表面增强激光解吸电离飞行时间质(surface enhanced laser desorption/ionization-time of flight-massspectrometry, SELDI-TOF-MS)技术研究LBW营养状况，以期为LBW的临床治疗提供参考依据，尽快启动更为合理的治疗方案，改善其预后。方法：将采集的91份血清样本分为4组，其中LBW59例：早产低出生体重儿（PLBW）37例，足月低出生体重儿（MLBW）22例；正常出生体重新生儿（NBW）32例。采用表面增强激光解析电离飞行时间质谱（SELDI-TOF-MS）技术及金（Au）芯片采集各组血清蛋白质谱指纹图，并用Biomarker Wizard软件筛选出差异蛋白。将所检测到有显著差异蛋白质峰质荷比（M/Z）输入蛋白质数据库(Swiss蛋白数据库http//us.expasy.org/tools/tagident.html)，得到与这些蛋白质峰质荷比（M/Z）相对应的蛋白质。以此分析和研究LBW营养状况及其蛋白质代谢特征。结果：低出生体重新生儿组与正常出生体重组有显著的统计学意义的蛋白质峰有150个（P0.01）。其中在LBW中低表达的蛋白质峰有98个，高表达的蛋白质峰有52个。从分析的结果看最有研究价值（峰值较高，，标准差小）的蛋白质峰有7个，其质荷比（M/Z）分别为2114.94Da、2381.06Da、6835.71Da、9444.83Da、4216.16Da、10421.72Da、5358.07Da。早产低出生体重儿组与足月低出生体重儿组有显著的统计学意义的蛋白质峰有74（P0.01）。其中在PLBW中高表达的蛋白质峰有64个，低表达的蛋白质峰有10个。从分析的结果看最有研究价值（峰值较高，标准差小）的蛋白质峰有5个，其质荷比（M/Z）分别为2074.52Da、2569.64Da、5349.36Da、5084.58Da、9096.87Da。结论:1、通过本次试验得出低出生体重新生儿有蛋白质代谢紊乱。2、低出生体重新生儿可能由于体内蛋白质的表达缺损或不平衡造成某些蛋白含量较正常出生体重新生儿低或高。早产低出生体重儿可能由于体内蛋白质的表达缺损或不平衡造成某些蛋白含量较足月低出生体重儿高或低。3、利用蛋白质芯片质谱技术研究低出生体重新生儿蛋白代谢特征可能为低出生体重新生儿的临床治疗提供参考依据。
[Abstract]:Objective: low birth weight infant, low birth weight (LBW) is defined as less than 2500g of birth weight within 1 hour. Small for gestational age, SGAA (small for gestational age, SGAA) is also called intrauterine growth retardation (IUGRG). In this study, surface enhanced laser desorption ionization enhanced laser desorption/ionization-time of flight-mass spectroscopy (SELDI-TOF-MS) technique was used to study the nutritional status of LBW in order to provide reference for clinical treatment of LBW, to initiate more reasonable treatment scheme and to improve its prognosis as soon as possible. Methods: 91 serum samples were divided into 4 groups, including 59 cases of LBW, 37 cases of premature low birth weight infants, 22 cases of full term low birth weight infants and 32 cases of normal birth weight newborns. Surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) technique and gold Au-chip were used to collect the fingerprint of serum proteins in each group, and the differential proteins were screened by Biomarker Wizard software. The protein peak-to-mass ratio (P / Z) of these proteins was input into Swiss protein database httpP / us.expasy.org.toolsrtagident.htmln, and the corresponding proteins were obtained. The nutritional status and protein metabolic characteristics of LBW were analyzed and studied. Results: there were 150 protein peaks in low birth weight newborn group and normal birth weight group. There were 98 protein peaks in LBW and 52 protein peaks in high expression. The results show that there are 7 protein peaks with high peak value and low standard deviation, and the ratio of mass to charge is 2114.94 DaC2381.06DaC6835.71Da9444.83Da4216.16DaP10421.72Da5358.07Da. The protein peak of low birth weight preterm infants and term low birth weight infants was 74 (P 0. 01) and that of full term low birth weight infants (P < 0. 01) was significantly higher than that of term low birth weight infants (P < 0. 01). There were 64 high expression protein peaks and 10 low expression protein peaks in PLBW. The results show that there are 5 protein peaks with high peak value and low standard deviation, and the ratio of mass to charge is 2074.52 Da1 2569.64 Da1 5349.36 Da1 5084.58 Da1 9096 87 Da. the results show that there are 5 peaks of protein with high peak value and low standard deviation, and the ratio of mass to charge is 2074.52 Da1, 2569.64 Da1, 5349.36 Da1, 5084.58 Da1. Conclusion: 1. By this experiment, we can conclude that low birth weight newborns have protein metabolism disorder. Low birth weight newborns may have lower or higher protein content than normal birth weight newborns because of protein expression defect or imbalance. The protein content of premature low birth weight infants may be higher or lower than that of full-term low birth weight infants due to protein expression defect or imbalance. Protein chip mass spectrometry was used to study proteins in low birth weight neonates. Metabolic characteristics may provide reference for the clinical treatment of low birth weight newborns.
【学位授予单位】：泸州医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R722.1

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