多重聚合酶链反应系统用于儿童常见细菌及真菌血流感染的价值研究

发布时间：2018-07-09 11:02

本文选题：多重聚合酶链反应系统 + 儿童血流感染　；参考：《中华医院感染学杂志》2017年01期

【摘要】：目的探讨多重聚合酶链反应系统用于儿童常见细菌及真菌血流感染的价值。方法选取2014年1月-2016年1月医院住院疑诊血流感染患儿135例,采集患儿的血标本,分别采用血培养法、16SrDNA-PCR和多重聚合酶链反应系统进行检测,对比3种方法检测儿童血流感染的阳性率。结果血培养法检测阳性标本12份,阳性率为8.89%,16SrDNA-PCR检测阳性标本26份,阳性率为19.26%,多重聚合酶链反应系统检测阳性标本24份,阳性率为17.78%;16SrDNA-PCR和多重聚合酶链反应系统的阳性率均显著高于血培养法(P0.05);16SrDNA-PCR和多重聚合酶链反应系统阳性率比较,差异无统计学意义。结论多重聚合酶链反应系统具有操作简单、检测时间短等优势,可在较短时间内鉴定儿童血流感染的常见病原菌,对临床诊疗有较好的指导意义。
[Abstract]:Objective to evaluate the value of multiplex polymerase chain reaction (PCR) in common bacterial and fungal blood stream infections in children. Methods 135 children with suspected blood flow infection were collected from January 2014 to January 2016. Blood samples were collected and detected by 16s rDNA-PCR and multiplex polymerase chain reaction (PCR). The positive rate of blood stream infection in children was detected by three methods. Results there were 12 positive specimens detected by blood culture, the positive rate was 8.89rDNA-PCR in 26 samples, the positive rate was 19.26, and the multiplex polymerase chain reaction system was used to detect 24 positive specimens. The positive rates of 16s rDNA-PCR and multiplex polymerase chain reaction were significantly higher than those of blood culture (P0.05) and multiplex polymerase chain reaction (MPCR). Conclusion the multiplex polymerase chain reaction system has the advantages of simple operation and short detection time. It can be used to identify common pathogenic bacteria of blood flow infection in children in a short time, which has a good guiding significance for clinical diagnosis and treatment.
【作者单位】：河南科技大学一附院检验科;
【基金】：河南杰出人才创新基金资助项目(0622345811)
【分类号】：R440;R725.1

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