β整合素家族在儿童急性T淋巴细胞白血病的表达及其临床意义
发布时间：2018-08-22 15:31
【摘要】：目的探讨β整合素家族成员在儿童急性T淋巴细胞白血病(T-ALL)的表达及临床意义。方法收集22例初诊T-ALL患儿和21例对照(非恶性血液病患者和骨髓移植供者)的骨髓标本,采用实时荧光定量PCR方法,检测β整合素家族各成员的m RNA表达;并利用整合素抑制剂精氨酸-甘氨酸-天冬氨酸(RGD)肽作用于Jurkat细胞,采用CCK 8法和流式细胞术检测Jurkat细胞生存率和凋亡情况。结果与对照组相比,T-ALL患儿的整合素β_2、β_3、β_5的m RNA表达水平显著下调(P0.05)。在外周血白细胞计数100×10~9/L、第33天骨髓不缓解或MRD阳性的T-ALL患儿中,整合素β_3表达水平较高(P0.05);复发T-ALL患儿整合素β_5的表达高于无复发T-ALL患儿(P0.05)。整合素β3高表达T-ALL患儿的EFS率、OS率均低于β_3低表达者(P0.05)。与未处理组比较,RGD肽处理的Jurkat细胞生存率较低、凋亡率均高(P0.05)。结论β整合素可能通过影响细胞的增殖和凋亡而影响T-ALL的发生发展,整合素β_5的表达与T-ALL复发风险密切相关,整合素β_3的表达与T-ALL患儿治疗反应及预后密切相关。
[Abstract]:Objective to investigate the expression and clinical significance of 尾 integrin family members in children with acute T lymphocyte leukemia (T-ALL). Methods Bone marrow samples were collected from 22 newly diagnosed children with T-ALL and 21 controls (non-malignant hematological patients and bone marrow transplant donors). The expression of m RNA in 尾 integrin family members was detected by real-time quantitative PCR. Jurkat cells were treated with integrin inhibitor arginine glycine aspartic acid (RGD) peptide. The survival rate and apoptosis of Jurkat cells were detected by CCK 8 method and flow cytometry. Results compared with the control group, the expression of integrin 尾 2, 尾 3, 尾 5 in children with T-ALL was significantly down-regulated (P0.05). The expression of integrin 尾 3 was higher in peripheral blood leukocyte count of 100 脳 10 ~ (9 / L) / L, and the expression of integrin 尾 _ (5) in children with recurrent T-ALL was higher than that in children without recurrence of T-ALL (P0.05), but the expression of integrin 尾 _ (3) was higher in children with unremitting bone marrow or positive MRD on the 33rd day (P0.05). The EFS rate and OS rate of T-ALL patients with high expression of integrin 尾 3 were lower than those with low expression of integrin 尾 3 (P0.05). Compared with the untreated group, the survival rate of Jurkat cells treated with RGD peptide was lower and the apoptosis rate was higher (P0.05). Conclusion 尾 integrin may affect the development of T-ALL by affecting cell proliferation and apoptosis. The expression of integrin 尾 5 is closely related to the risk of recurrence of T-ALL. The expression of integrin 尾 3 is closely related to the therapeutic response and prognosis of children with T-ALL.
【作者单位】： 重庆医科大学附属儿童医院血液肿瘤科/儿童发育疾病研究教育部重点实验室/儿科学重庆市重点实验室/儿童发育重大疾病国家国际科技合作基地;
【基金】：重庆市卫生局课题(2015MSXM031)
【分类号】：R733.71
，
本文编号：2197532
[Abstract]:Objective to investigate the expression and clinical significance of 尾 integrin family members in children with acute T lymphocyte leukemia (T-ALL). Methods Bone marrow samples were collected from 22 newly diagnosed children with T-ALL and 21 controls (non-malignant hematological patients and bone marrow transplant donors). The expression of m RNA in 尾 integrin family members was detected by real-time quantitative PCR. Jurkat cells were treated with integrin inhibitor arginine glycine aspartic acid (RGD) peptide. The survival rate and apoptosis of Jurkat cells were detected by CCK 8 method and flow cytometry. Results compared with the control group, the expression of integrin 尾 2, 尾 3, 尾 5 in children with T-ALL was significantly down-regulated (P0.05). The expression of integrin 尾 3 was higher in peripheral blood leukocyte count of 100 脳 10 ~ (9 / L) / L, and the expression of integrin 尾 _ (5) in children with recurrent T-ALL was higher than that in children without recurrence of T-ALL (P0.05), but the expression of integrin 尾 _ (3) was higher in children with unremitting bone marrow or positive MRD on the 33rd day (P0.05). The EFS rate and OS rate of T-ALL patients with high expression of integrin 尾 3 were lower than those with low expression of integrin 尾 3 (P0.05). Compared with the untreated group, the survival rate of Jurkat cells treated with RGD peptide was lower and the apoptosis rate was higher (P0.05). Conclusion 尾 integrin may affect the development of T-ALL by affecting cell proliferation and apoptosis. The expression of integrin 尾 5 is closely related to the risk of recurrence of T-ALL. The expression of integrin 尾 3 is closely related to the therapeutic response and prognosis of children with T-ALL.
【作者单位】： 重庆医科大学附属儿童医院血液肿瘤科/儿童发育疾病研究教育部重点实验室/儿科学重庆市重点实验室/儿童发育重大疾病国家国际科技合作基地;
【基金】：重庆市卫生局课题(2015MSXM031)
【分类号】：R733.71
，
本文编号：2197532
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