氢化可的松对慢性功能性内脏痛的抑制作用

发布时间：2018-08-25 15:45

【摘要】：目的探究氢化可的松(HYD)对新生期结直肠扩张所致的慢性功能性内脏痛大鼠的痛敏反应的影响及其可能的作用途径。方法SD大鼠出生后第8~14天行结直肠扩张,8周龄后采用腹壁撤退反射评分(AWR)和痛阈测定筛选出具有慢性内脏痛的肠易激综合征(IBS)大鼠,并随机分为4组:生理盐水组、低剂量、中剂量和高剂量HYD组;未经结直肠扩张处理的大鼠为对照组。通过AWR和痛阈测定尾静脉注射HYD前后大鼠痛敏反应程度变化;采用酶联免疫吸附法测定大鼠血清中皮质醇的含量及脊髓胸腰段和腰骶段内啡肽、脑啡肽的含量;行旷场实验测定大鼠心理焦虑程度。结果(1)IBS组大鼠血清中皮质醇的含量显著低于对照组大鼠(P0.05);(2)IBS组大鼠注射中剂量和低剂量HYD后痛阈均值比注射前显著升高(P0.05);(3)与生理盐水组比较,低剂量HYD组大鼠脊髓胸腰段内啡肽、脑啡肽含量显著升高(P0.05);(4)旷场实验显示,IBS组大鼠注射低剂量HYD后大鼠运动总路程增加,平均速度加快(P0.05)。结论一定剂量的HYD能减轻大鼠的痛敏反应和焦虑抑郁行为,其机制可能与脊髓胸腰段内啡肽、脑啡肽含量变化有关。
[Abstract]:Objective to investigate the effect of hydrocortisone (HYD) on the hypersensitivity of chronic functional visceral pain induced by colorectal dilatation in neonatal rats and its possible mechanism. Methods SD rats with irritable bowel syndrome (IBS) with chronic visceral pain were selected by abdominal retreat reflex score (AWR) and pain threshold measurement after 8 weeks of colorectal dilatation on the 814th day after birth. The rats were randomly divided into 4 groups: normal saline group, low dose. Middle and high dose HYD groups and control group without colorectal dilatation. AWR and pain threshold were used to determine the degree of pain sensitivity before and after HYD injection, and the contents of cortisol in serum, endorphin and enkephalin in thoracolumbar and lumbosacral segments of spinal cord were measured by enzyme-linked immunosorbent assay (Elisa). The degree of psychological anxiety in rats was measured by open field experiment. Results (1) the content of cortisol in serum of IBS group was significantly lower than that of control group (P0.05); (2). The mean pain threshold after middle dose and low dose HYD injection in IBS group was significantly higher than that before injection (P0.05); (3). Compared with normal saline group, the low dose HYD group was significantly higher than that of normal saline group in the level of endorphin in thoracolumbar segment of spinal cord. The content of enkephalin increased significantly (P0.05); (4) Open field experiment showed that the total distance of movement increased and the average speed increased after low dose HYD injection (P0.05). Conclusion certain dose of HYD can reduce the pain sensitivity and anxiety and depression in rats, and its mechanism may be related to the changes of the contents of endorphins and enkephalins in the thoracolumbar segment of the spinal cord.
【作者单位】：福建医科大学基础医学院生理学与病理生理学系疼痛研究室福建省神经胶质和疾病重点实验室;福建医科大学基础医学院;
【基金】：国家自然科学基金(81471138) 福建省自然科学基金(2014J01124) 国家级大学生创新创业训练计划项目(201510392023)
【分类号】：R722.1

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