RANKL、OPG在小儿骨瘤样病变（单纯性骨囊肿、动脉瘤样骨囊肿）组织中的表达研究

发布时间：2018-12-09 20:11

【摘要】：【目的】通过对小儿骨瘤样病变（单纯性骨囊肿、动脉瘤样骨囊肿）组织中OPG、RANKL蛋白表达水平的检测，并与正常的骨组织作对照，分析这两种蛋白在不同溶骨性病变中的表达是否存在差异性及意义。【方法】共收集动脉瘤样骨囊肿标本23例、单纯性骨囊肿标本31例，正常骨组织标本14例。采用免疫组化SABC方法研究单纯性骨囊肿、动脉瘤样骨囊肿中OPG、 RANKL的表达并分析其差异性。应用Imagepro-Plus6.0图像处理软件及数据处理软件SPSS17.0对图像及所得数据经行分析。【结果】在单纯性骨囊肿及动脉瘤样骨囊肿中，RANKL蛋白的表达量明显高于正常骨组织的表达,具有统计学意义，其中动脉瘤样骨囊肿RANKL蛋白的表达量较单纯性骨囊肿增高，有统计学意义；两种瘤样病变中OPG蛋白的表达量低于正常骨组织的表达，有统计学意义，其中动脉瘤样骨囊肿OPG稍高于单纯性骨囊肿，无统计学意义。RANKL/OPG比值在动脉瘤样骨囊肿＞单纯性骨囊肿㧐正常组织，有统计学意义。【结论】RANKL、OPG平衡的严重破坏是导致机体发生自发性骨质破坏的原因；RANKL/OPG比值的轻微升高不会导致机体发生溶骨性破坏，推测RANKL/OPG比值达到一定界点后才会导致机体发生骨破坏，，此比值界点有待进一步研究。初步表明RANKL的高表达与动脉瘤样骨囊肿的复发密切相关。
[Abstract]:[objective] to detect the expression of OPG,RANKL protein in osteomatous lesions (simple bone cysts, aneurysm-like bone cysts) of children, and to compare it with normal bone tissues. To analyze the difference and significance of the expression of these two proteins in different osteolytic lesions. [methods] 23 cases of aneurysm like bone cyst, 31 cases of simple bone cyst and 14 cases of normal bone tissue were collected. Immunohistochemical SABC method was used to study the expression of OPG, RANKL in simple bone cysts and aneurysm like bone cysts. Imagepro-Plus6.0 image processing software and data processing software SPSS17.0 are used to analyze the image and the data. [results] the expression of RANKL protein in simple bone cysts and aneurysm like bone cysts was significantly higher than that in normal bone tissues, and the expression of RANKL protein in aneurysm like bone cysts was higher than that in simple bone cysts. Statistical significance; The expression of OPG protein in two kinds of tumor-like lesions was lower than that in normal bone tissue, and the expression of OPG in aneurysm-like bone cysts was slightly higher than that in simple bone cysts. There was no statistical significance. The ratio of RANKL/OPG in aneurysm like bone cyst was higher than that in simple bone cyst. Normal tissue, with statistical significance. [conclusion] the serious destruction of RANKL,OPG balance is the cause of spontaneous bone destruction. The slight increase of RANKL/OPG ratio will not lead to osteolytic destruction. It is speculated that bone destruction will occur only when the RANKL/OPG ratio reaches a certain point, which should be further studied. The high expression of RANKL is closely related to the recurrence of aneurysm like bone cyst.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R738.1

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