新生儿高胆红素血症与先天性巨细胞病毒感染的相关性研究
发布时间：2018-12-30 17:55
【摘要】：目的： 1、探讨新生儿高胆红素血症与巨细胞病毒(HCMV)感染的相关性及临床意义。 2、进一步探讨荧光定量核酸扩增法对新生儿巨细胞病毒感染的诊断价值。 材料和方法： 收集淄博市妇幼保健院不明原因新生儿高胆红素血症标本170例(排除高胆红素血症新生儿血清标本100例为正常对照),疾病诊断符合《实用新生儿学》新生儿高胆红素血症诊断标准,标本分离血浆后在全自动生化分析以上检测肝功能,记录肝功各项指标结果,记录胆红素浓度；以酶联免疫吸附法(ELISA)检测受检者血浆中HCMV-IgM和HCMV-IgG抗体,荧光定量PCR方法检测血浆游离DNA浓度和单个核细胞DNA浓度,并进行统计学处理。 结果： 1、新生儿高胆红素血症患者标本HCMV-IgM和HCMV-IgG阳性率均高于正常对照组,两者比较有统计学意义(P0.05)。 2、单个核细胞中HCMVDNA阳性率较血浆游离DNA高,两者比较有统计学意义(P0.05)。 3、HCMV-IgG阳性组肝功能指标ALT、AST、GGT均高于HCMV-IgG阴性组,两者比较有统计学意义(P0.05)。 结论： 新生儿高胆红素血症患者血清HCMV-IgG及HCMV-IgM阳性率均高于正常对照组,两者比较有统计学意义,说明新生儿的巨细胞病毒感染与新生儿高胆红素血症及肝损伤相关性大。单个核细胞中检测HCMV-DNA阳性率高于血浆中的游离DNA,两者比较有统计学意义,说明在进行HCMV的DNA检测时,宜选用单个核细胞。
[Abstract]:Objective: 1 to investigate the relationship between neonatal hyperbilirubinemia and cytomegalovirus (HCMV) infection and its clinical significance. 2. To investigate the diagnostic value of fluorescent quantitative nucleic acid amplification in neonatal cytomegalovirus infection. Materials and methods: 170 samples of neonatal hyperbilirubinemia with unknown cause were collected from Zibo Maternal and Child Health Hospital (excluding 100 serum samples of neonates with hyperbilirubinemia as normal control). The diagnosis of the disease was in accordance with the diagnostic criteria of neonatal hyperbilirubinemia. After the plasma samples were separated, the liver function was detected by automatic biochemical analysis, the results of various indexes of liver function were recorded, and the concentration of bilirubin was recorded. Enzyme linked immunosorbent assay (ELISA) was used to detect the HCMV-IgM and HCMV-IgG antibodies in the plasma of the subjects, and fluorescence quantitative PCR was used to detect the concentration of free DNA and DNA in mononuclear cells. Results: 1. The positive rates of HCMV-IgM and HCMV-IgG in neonates with hyperbilirubinemia were significantly higher than those in normal controls (P0.05). 2. The positive rate of HCMVDNA in mononuclear cells was higher than that in plasma free DNA (P0.05). 3The liver function index ALT,AST,GGT of HCMV-IgG positive group was significantly higher than that of HCMV-IgG negative group (P0.05). Conclusion: the positive rates of serum HCMV-IgG and HCMV-IgM in neonates with hyperbilirubinemia were significantly higher than those in normal controls. The results showed that neonatal cytomegalovirus infection was associated with neonatal hyperbilirubinemia and liver injury. The positive rate of HCMV-DNA in mononuclear cells was higher than that of free DNA, in plasma, which indicated that mononuclear cells should be selected for DNA detection of HCMV.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R722.1
本文编号：2395927
[Abstract]:Objective: 1 to investigate the relationship between neonatal hyperbilirubinemia and cytomegalovirus (HCMV) infection and its clinical significance. 2. To investigate the diagnostic value of fluorescent quantitative nucleic acid amplification in neonatal cytomegalovirus infection. Materials and methods: 170 samples of neonatal hyperbilirubinemia with unknown cause were collected from Zibo Maternal and Child Health Hospital (excluding 100 serum samples of neonates with hyperbilirubinemia as normal control). The diagnosis of the disease was in accordance with the diagnostic criteria of neonatal hyperbilirubinemia. After the plasma samples were separated, the liver function was detected by automatic biochemical analysis, the results of various indexes of liver function were recorded, and the concentration of bilirubin was recorded. Enzyme linked immunosorbent assay (ELISA) was used to detect the HCMV-IgM and HCMV-IgG antibodies in the plasma of the subjects, and fluorescence quantitative PCR was used to detect the concentration of free DNA and DNA in mononuclear cells. Results: 1. The positive rates of HCMV-IgM and HCMV-IgG in neonates with hyperbilirubinemia were significantly higher than those in normal controls (P0.05). 2. The positive rate of HCMVDNA in mononuclear cells was higher than that in plasma free DNA (P0.05). 3The liver function index ALT,AST,GGT of HCMV-IgG positive group was significantly higher than that of HCMV-IgG negative group (P0.05). Conclusion: the positive rates of serum HCMV-IgG and HCMV-IgM in neonates with hyperbilirubinemia were significantly higher than those in normal controls. The results showed that neonatal cytomegalovirus infection was associated with neonatal hyperbilirubinemia and liver injury. The positive rate of HCMV-DNA in mononuclear cells was higher than that of free DNA, in plasma, which indicated that mononuclear cells should be selected for DNA detection of HCMV.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R722.1
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