骨巨细胞瘤MRI表现与骨质破坏相关蛋白表达的初步研究

发布时间：2018-03-29 03:26

本文选题：骨巨细胞瘤　切入点：NF-kB　出处：《临床放射学杂志》2015年05期

【摘要】：目的研究骨巨细胞瘤(GCT)骨皮质破坏与肿瘤组织中P65、RANKL、OPG、MMP-9表达程度的关系,初步探讨MRI所示的侵袭性征象所代表的生物学行为。方法搜集2010年8月至2012年1月本院诊治的28例GCT患者,根据MRI表现有无骨皮质明显中断和软组织肿块形成分为2组,运用半定量逆转录聚合酶链式反应(RT-PCR)的方法检测各个肿瘤组织中P65、RANKL、OPG、MMP-9的mRNA的表达程度,分析2组间P65、RANKL、OPG、MMP-9的mRNA的表达程度有无差异以及各种蛋白的mRNA表达间有无相关性。所得的数据采用SPSS 13.0统计软件,采用秩和检验法和相关分析法,以P≤0.05为差异有统计学意义。结果有骨皮质明显中断和软组织肿块形成的GCT较无骨皮质明显中断和软组织肿块形成的GCT的P65、RANKL、MMP-9的表达量升高,OPG的表达无统计学差异。结论MRI表现有骨皮质中断征象的GCT,其骨质破坏蛋白表达增加,提示GCT中骨质破坏活动增加。
[Abstract]:Objective to study the relationship between the cortical destruction of giant cell tumor of bone (GCT) and the expression level of P65 RANKL OP-GG MMP 9 in tumor tissues, and to explore the biological behavior represented by the invasive signs shown by MRI. Methods from August 2010 to January 2012, 28 patients with GCT were enrolled in our hospital. According to MRI findings, bone cortical disruption and soft tissue mass formation were divided into two groups. Semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of P65-RANKL- OP-GG MMP-9 in various tumor tissues. To analyze the difference of mRNA expression of P65 RANKL OP-GG p9 between two groups and the correlation of mRNA expression of various proteins, the data were analyzed by SPSS 13.0 statistical software, rank sum test and correlation analysis. Results GCT with obvious cortical disruption and soft tissue mass formation were significantly higher than those with GCT without cortical disruption and soft tissue mass formation. There was no significant difference in the expression of P65 RANKLMP-9 between the two groups. Conclusion GCTs with cortical disruption were found in MRI, and the expression of bone destruction protein was increased in GCTs. The results suggest that the activity of bone destruction is increased in GCT.
【作者单位】：浙江大学医学院附属第二医院放射科;上海交通大学医学院附属瑞金医院放射科;
【分类号】：R738.1;R445.2

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