低频超声联合微泡增强宫颈癌Hela细胞对顺铂敏感性的实验研究

发布时间：2017-12-31 04:37

本文关键词：低频超声联合微泡增强宫颈癌Hela细胞对顺铂敏感性的实验研究　出处：《第三军医大学学报》2016年15期 　论文类型：期刊论文

【摘要】：目的研究低频率、低声压超声激励微泡增强顺铂对宫颈癌Hela细胞的杀伤作用。方法实验分为对照组(C组),顺铂组(P组),顺铂联合超声组(PU组),超声微泡组(UM组),超声微泡联合顺铂组(PUM组)。采用频率1MHz、声压400 k Pa的非聚焦超声联合脂质微泡及顺铂,按照分组处理对数期生长的宫颈癌Hela细胞。Hoechst33342染色后通过荧光显微镜观察并计算各组细胞的凋亡率;采用Cell Counting Kit-8(CCK-8)法检测处理后各组细胞的增殖活性;对各组细胞行Annexin V-FITC/PI双染色后,运用流式细胞仪检测各组处理后24 h细胞凋亡情况。结果测定Hela细胞对顺铂的敏感性,顺铂的IC50为4.882μg/m L。通过流式细胞仪检测到超声微泡顺铂组(PUM)的细胞凋亡率为58.1%,较顺铂联合超声组(PU)增高40%,明显高于其余各组,组间差异有统计学意义(P0.05);CCK-8检测结果显示PUM组较其余各组细胞生长明显受到抑制;Hoechst33342染色后,PUM组典型凋亡细胞明显多于其余各组。结论低频率低声压超声联合微泡可以增强顺铂对宫颈癌Hela细胞的杀伤作用,明显抑制Hela细胞生长,促进其凋亡。
[Abstract]:Objective to study the cytotoxicity of cisplatin on Hela cells induced by low frequency and low pressure ultrasound. Methods the mice were divided into control group (C group) and cisplatin group (P group). Cisplatin combined with ultrasound group (pu group), ultrasonic microbubble group (group UM), ultrasonic microbubble group (group Pu) and cisplatin group (group Pum) were used at a frequency of 1 MHz. The sound pressure was 400 KPA unfocused ultrasound combined with lipid microbubbles and cisplatin. The apoptosis rate of cervical cancer Hela cell line. Hoechst33342 was observed by fluorescence microscope and the apoptosis rate of each group was calculated. The proliferation activity of each group was detected by Cell Counting Kit-8 CCK-8 method. Annexin V-FITC / Pi double staining was performed on the cells in each group. Flow cytometry was used to detect the apoptosis of Hela cells 24 hours after treatment. Results the sensitivity of Hela cells to cisplatin was determined. The IC50 of cisplatin was 4.882 渭 g / mL. The apoptosis rate of Cisplatin group was 58.1% by flow cytometry. Compared with Cisplatin combined with ultrasound group, it increased by 40%, which was significantly higher than that in other groups, and the difference between groups was statistically significant (P 0.05). The results of CCK-8 showed that the growth of cells in PUM group was significantly inhibited than that in other groups. After Hoechst33342 staining. Conclusion low frequency low frequency ultrasound combined with microbubbles can enhance the killing effect of cisplatin on cervical cancer Hela cells and inhibit the growth of Hela cells. Promote its apoptosis.
【作者单位】：安徽医科大学解放军306临床学院;第三军医大学新桥医院妇产科;第三军医大学新桥医院超声科;
【基金】：国家自然基金面上项目(81227004)~~
【分类号】：R737.33
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