4.1N蛋白抑制人卵巢透明细胞癌细胞迁移侵袭及其机制初探

发布时间：2018-01-13 23:33

本文关键词：4.1N蛋白抑制人卵巢透明细胞癌细胞迁移侵袭及其机制初探　出处：《现代妇产科进展》2015年05期 　论文类型：期刊论文

【摘要】：目的:探讨4.1N蛋白对卵巢透明细胞癌细胞迁移和侵袭能力的调控并初步探讨相关分子机制。方法:应用免疫组化法检测4.1N蛋白在卵巢透明细胞癌组织中的表达缺失状况。建立4.1N稳定转染卵巢透明细胞癌ES-2细胞系,通过划痕实验、Transwell迁移和侵袭实验观察细胞迁移侵袭能力的改变。利用qRT-PCR和Western blot法检测紧密连接相关分子(Claudin-4和ZO-1)、上皮-间质转化(EMT)标记物(E-cadherin、Ncadherin和Vimentin)和相关转录因子(Twist、Slug和ZEB-2)及基质金属蛋白酶(MMP-2和MMP-3)表达水平的变化。结果:与卵巢异位的子宫内膜组织相比,卵巢透明细胞癌中4.1N蛋白表达水平显著降低甚至缺失(P0.0001)。过表达4.1N后,ES-2细胞的迁移和侵袭能力显著降低,Claudin-4和ZO-1表达上调,Twist、Slug、ZEB-2、MMP-2和MMP-3表达水平下降。结论:4.1N在卵巢透明细胞癌细胞中可能通过调控紧密连接蛋白、部分上皮-间质转化转录因子和基质金属蛋白酶的表达水平,抑制癌细胞的迁移和侵袭能力,进而参与负性调控卵巢透明细胞癌的演进。
[Abstract]:Objective: to investigate the regulation of 4.1 N protein on the migration and invasion of ovarian clear cell carcinoma cells and to explore the molecular mechanism. The expression of 4.1N protein in clear cell carcinoma of ovary was detected by immunohistochemical method. The ES-2 cell line of ovarian clear cell carcinoma was established by 4.1N stable transfection. Through the scratch test. Transwell migration and invasion assay were used to observe the change of cell migration and invasion ability. QRT-PCR and Western blot were used to detect the tightly linked molecules (. Claudin-4 and ZO-1). E-cadherin and Vimentin (E-cadherin and Vimentin) and related transcription factor Twist. Slug and ZEB-2) and matrix metalloproteinase (MMP-2) and MMP-3) expression levels. Results: compared with ovarian ectopic endometrium tissue. The expression level of 4.1N protein in ovarian clear cell carcinoma was significantly decreased or even deleted P0.0001N, and the migration and invasion ability of Ovarian clear cell carcinoma was significantly decreased after the expression of 4.1 N. The expression of Claudin-4 and ZO-1 up-regulated the expression of Twistus Slugfus ZEB-2. Conclusion the expression of MMP-2 and MMP-3 in ovarian clear cell carcinoma cells may be regulated by tight junction protein. The expression of transcriptional factors and matrix metalloproteinases (MMPs) in some epithelium-mesenchymal cells inhibits the migration and invasion of cancer cells and thus participates in the negative regulation of the progression of ovarian clear cell carcinoma.
【作者单位】：北京大学医学部基础医学院病理系;北京大学医学部基础医学院解剖与组胚系;红细胞生理实验室纽约血液中心;郑州科技大学生物工程系;
【分类号】：R737.31
【正文快照】： 上皮性卵巢癌(epithelial ovarian cancer,EOC)是女性常见的恶性肿瘤之一,死亡率居女性生殖道恶性肿瘤首位[1]。作为I型EOC的一种特殊亚型,透明细胞癌的发病机制及预后一直备受关注。2012年FIGO正式把异位子宫内膜(endometriosis,EMs)列为透明细胞癌的前驱病变。然而,对于通常

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