囊胚内细胞团和滋养外胚层基因表达研究

发布时间：2018-01-14 11:38

  本文关键词：囊胚内细胞团和滋养外胚层基因表达研究　出处：《中华生殖与避孕杂志》2017年09期 　论文类型：期刊论文

  更多相关文章： 囊胚 内细胞团 滋养外胚层 基因 单细胞测序

【摘要】：目的研究人胚胎植入前囊胚内细胞团和滋养外胚层细胞基因的表达。方法收集辅助生殖来源人受精后6 d Gardner评分5AA囊胚,显微镜下微吸管机械分离内细胞团和滋养外胚层细胞,单细胞测序检测,选取筛选的差异表达基因并进行无监督层次聚类分析和Gene Ontology(GO)功能分类分析。结果对3枚受精后6 d的5AA囊胚内细胞团14个单细胞和滋养外胚层19个单细胞测序,结果显示内细胞团1 283个基因表达上调,其G O生物学功能主要是参与DNA依赖的转录、生物种间相互作用、抗原加工和表达、免疫反应、信号转导、氧化还原、细胞分化、抗凋亡、神经系统发育、细胞黏附等,参与丝裂原活化蛋白激酶(MAPKs)信号通路、肌动蛋白细胞骨架、黏附、轴引导、Jak-STAT、Wnt信号通路。滋养外胚层1 073个基因表达上调,其GO生物学功能主要是参与转录、DNA依赖的转录、细胞周期、蛋白分解代谢、蛋白氨基酸磷酸化、蛋白质运输、细胞分裂、有丝分裂、泛素依赖性蛋白分解、细胞内蛋白质转运等;参与泛素介导蛋白水解、鞘脂代谢、缬氨酸,亮氨酸和异亮氨酸降解、果糖和甘露糖代谢、氨基磷酸酯代谢、类固醇的生物合成、抗原处理和表达等信号通路。结论从空间维度揭示囊胚内细胞团和滋养外胚层的基因表达,显示两者相互协调,精细调节囊胚发育和胚胎植入过程,进一步数据分析有望寻找到调控胚胎植入的内源性关键特异分子。
[Abstract]:Objective to study the gene expression of cell mass and trophodermal cell in preimplantation blastocyst. Methods 5AA blastocysts were collected on the 6th day after assisted reproduction. Micropipette mechanical isolation of inner cell mass and trophoblast ectodermal cells were examined by single cell sequencing. The differentially expressed genes were selected and analyzed by unsupervised hierarchical clustering and Gene Ontology (GOG). Results three 5AA blastocysts from 6 days after fertilization were sequenced by 14 single cells and 19 single cells in the trophodermal layer. The results showed that 1,283 genes were up-regulated in the inner cell mass, and their biological functions were mainly involved in DNA dependent transcription, interspecies interaction, antigen processing and expression, and immune response. Signal transduction, redox, cell differentiation, anti-apoptosis, nervous system development, cell adhesion, mitogen-activated protein kinase (MAPKs) signaling pathway, actin cytoskeleton, adhesion. The expression of 1073 genes was up-regulated in the trophodermal layer. Its go biological function was mainly involved in transcription of DNA dependent transcription and cell cycle. Protein catabolism, protein amino acid phosphorylation, protein transport, cell division, mitosis, ubiquitin dependent protein decomposition, intracellular protein transport, etc. Participate in ubiquitin mediated protein hydrolysis, sphingolipid metabolism, valine, leucine and isoleucine degradation, fructose and mannose metabolism, aminophosphate metabolism, steroid biosynthesis. Conclusion the gene expression in blastocyst and trophodermal layer is revealed from the spatial dimension, which shows that the two genes coordinate with each other and regulate the development of blastocyst and embryo implantation. Further data analysis is expected to find key endogenous specific molecules regulating embryo implantation.
【作者单位】： 北京积水潭医院妇产科;
【基金】：国家自然科学基金面上项目(81070493)~~
【分类号】：R714.8
【正文快照】： 哺乳动物胚胎早期发育具有自我调控能力,胚胎没有极化之前,改变卵裂球的空间位置,或者移除一个卵裂球,胚胎都能正常发育。胚胎逐渐分裂和发育,细胞开始具有极性和特异性,这种分化发育可以追溯到胚胎发育早期。对小鼠4-细胞胚胎研究证实[1],细胞具有发育特异性,细胞质成分差异，

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