维生素C及其转运体SVCT在子痫前期发病机制中的作用研究

发布时间：2018-01-14 18:05

本文关键词：维生素C及其转运体SVCT在子痫前期发病机制中的作用研究　出处：《实用妇产科杂志》2016年04期 　论文类型：期刊论文

【摘要】：目的:通过前瞻性随机对照临床研究,探讨围生期维生素C的摄入及代谢与子痫前期(PE)的关系,探索PE的可能发病机制。方法:随机选取2011年9月至2014年3月广州医科大学附属第三医院住院孕妇344例,包括研究组(PE患者)170例、对照组(正常妊娠)174例。记录每日维生素C摄入量;收集其清晨中段尿、外周血、脐血及胎儿附属物(胎盘、胎膜、脐带),采用ELISA方法检测母体尿液、外周血及脐血血清维生素C含量,免疫组化法观察胎盘、胎膜、脐带依赖钠离子维生素C转运蛋白(SVCT)1及SVCT2的表达情况,PCR-RFLP法检测SLC23a1的多态性。结果:1研究组每日维生素C摄入量及尿液维生素C含量与对照组相比,差异无统计学意义(P0.05);2研究组母体外周血维生素C含量明显高于对照组,差异有统计学意义(P0.05);3研究组脐血维生素C含量低于对照组,差异有统计学意义(P0.05);4研究组胎儿附属物SVCT1及SVCT2表达水平均较对照组低,差异有统计学意义(P0.05);5研究组与对照组SLC23a1多态性在rs35817838、rs33972313、rs6886922和rs34521685位点上并无差异(P0.05)。结论:胎儿附属物SVCT1及SVCT2表达水平降低,从而影响母体与胎儿之间的维生素C的转运,使得维生素C等抗氧化物不能有效到达胎盘,这可能是PE发病机制之一。
[Abstract]:Objective: to investigate the relationship between perinatal vitamin C intake and metabolism and preeclampsia (PEP) by prospective randomized controlled clinical study. Methods: from September 2011 to March 2014, 344 pregnant women in the third affiliated Hospital of Guangzhou Medical University were randomly selected. There were 170 patients with PE in the study group and 174 patients in the control group (normal pregnancy). Daily vitamin C intake was recorded. Urine, peripheral blood, umbilical cord blood and fetal appendages (placenta, fetal membrane, umbilical cord) were collected. Serum vitamin C levels in maternal urine, peripheral blood and umbilical cord blood were detected by ELISA method. The expression of SVCT-1 and SVCT2 in placenta, fetal membrane and umbilical cord dependent vitamin C transporter was observed by immunohistochemical method. Results the daily vitamin C intake and urine vitamin C content in the study group were compared with those in the control group. The difference was not statistically significant (P 0.05). 2the content of vitamin C in maternal peripheral blood of the study group was significantly higher than that of the control group, and the difference was statistically significant (P 0.05). 3The content of vitamin C in the study group was lower than that in the control group, and the difference was statistically significant (P 0.05). (4) the expression of SVCT1 and SVCT2 in the study group was lower than that in the control group (P 0.05). 5 the polymorphism of SLC23a1 in the study group and the control group was rs35817838 and rs33972313. There was no difference between rs6886922 and rs34521685 loci (P 0.05). Conclusion: the expression of SVCT1 and SVCT2 in fetal appendages is decreased. Thus affecting the transport of vitamin C between mother and fetus, vitamin C and other antioxidants can not reach the placenta effectively, which may be one of the pathogenesis of PE.
【作者单位】：广州医科大学附属第三医院广东省产科重大疾病重点实验室;
【基金】：广东省自然科学基金(编号:S2013010014810)
【分类号】：R714.244
【正文快照】： 子痫前期(preeclampsia,PE)发病率为9.4%,是导致全球孕产妇和围生儿发病及死亡的主要原因之一[1]。目前发病机制尚不明确,多数学者认为,其起源于胎盘的病理生理改变,胎盘浅着床及随之产生的氧化应激可能是PE发病的首要因素,而滋养细胞侵袭能力下降以及子宫螺旋动脉重铸障碍是P

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