皮秒脉冲电场对宫颈癌侵袭能力影响的实验研究

发布时间：2018-01-17 03:34

本文关键词：皮秒脉冲电场对宫颈癌侵袭能力影响的实验研究　出处：《解放军医学杂志》2015年09期 　论文类型：期刊论文

【摘要】：目的研究不同场强皮秒脉冲电场(ps PEF)对宫颈癌侵袭能力的影响。方法 24只BALB/c裸鼠建立宫颈癌荷瘤裸鼠模型,待肿瘤最大径长至0.8~1.0cm时将其随机均分为:对照组(不予脉冲电场处理)及低场强(50k V/cm)、中场强(60k V/cm)和高场强(70k V/cm)组(n=6),后3组分别给予场强ps PEF处理。1周后处死小鼠,行HE染色和电镜观察肿瘤的组织形态学变化,免疫组化染色检测肿瘤组织内血管内皮生长因子(VEGF)和基质金属蛋白酶-9(MMP-9)的表达,Western blotting分析VEGF和MMP-9蛋白水平的变化。结果 ps PEF处理后,HE染色观察到组织的坏死面积随脉冲电场强度的增加逐渐增大,电镜下可见随着脉冲电场强度的增加肿瘤细胞呈现出不同程度的凋亡和坏死。免疫组化检测结果显示,随着脉冲电场强度的增加,肿瘤组织内VEGF和MMP-9阳性细胞数逐渐减少,VEGF和MMP-9蛋白平均光密度值较对照组明显下降,且随着脉冲电场强度的增强,平均光密度值逐渐下降,组间差异有统计学意义(P0.05)。Western blotting结果显示,VEGF和MMP-9蛋白表达水平随着脉冲电场强度的增加逐渐降低,组间差异有统计学意义(P0.05)。结论 ps PEF具有抗宫颈癌效应,能抑制宫颈癌细胞分泌的VEGF和MMP-9,降低宫颈癌细胞的侵袭能力。
[Abstract]:Objective to study the effect of picosecond pulsed electric field (PEFs) on the invasive ability of cervical carcinoma in 24 BALB/c nude mice. When the maximum diameter of the tumor was 0.8 ~ 1.0cm, the tumor was randomly divided into two groups: control group (no pulsed electric field treatment) and low field intensity (50 kV / cm). 60 kV / cm in midfield and 70 kV / cm in high field. The mice in the latter three groups were treated with PS PEF for 1. 1 weeks. The histomorphologic changes of the tumor were observed by HE staining and electron microscope. The expression of VEGF and MMP-9 were detected by immunohistochemical staining. The changes of VEGF and MMP-9 protein levels were analyzed by Western blotting. He staining showed that the necrotic area of tissue increased with the increase of pulsed electric field intensity. With the increase of pulsed electric field intensity, the tumor cells showed different degrees of apoptosis and necrosis. The results of immunohistochemistry showed that with the increase of pulsed electric field intensity, the tumor cells showed different degrees of apoptosis and necrosis. The number of VEGF and MMP-9 positive cells in tumor tissues decreased gradually compared with the control group, and the average optical density of MMP-9 and VEGF protein decreased significantly compared with the control group, and increased with the intensity of pulsed electric field. The average optical density decreased gradually, and the difference between the two groups was statistically significant (P 0.05). Western blotting showed that there was no significant difference between the two groups. The expression of VEGF and MMP-9 protein decreased with the increase of pulsed electric field intensity, and the difference was statistically significant (P 0.05). Conclusion PS PEF has anti-cervical cancer effect. It can inhibit the secretion of VEGF and MMP-9 by cervical cancer cells, and reduce the invasion ability of cervical cancer cells.
【作者单位】：重庆医科大学附属第二医院妇产科;重庆大学输配电装备及系统安全与新技术国家重点实验室;
【基金】：国家自然科学基金青年基金(81301928,81201745) 重庆市卫生局医学科研项目(2012-2-068,2011-2-155)~~
【分类号】：R737.33
【正文快照】： 2 0 1 2年国际癌症研究机构公布的全球肿瘤流行病学统计数据表明,发展中国家宫颈癌新增病例44.45万,其中死亡病例23.02万,分别居所有新增病例和死亡病例的第2、3位[1]。虽然世界各国的研究人员都在致力于探索新的宫颈癌治疗策略[2-4],但是目前的手术治疗及放、化疗效果有限,且

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