脑源性神经营养因子对人滋养层细胞增殖和侵袭的影响及其机制

发布时间：2018-01-17 20:40

本文关键词：脑源性神经营养因子对人滋养层细胞增殖和侵袭的影响及其机制　出处：《山东医药》2017年35期 　论文类型：期刊论文

【摘要】：目的探讨脑源性神经营养因子(BDNF)对人滋养层细胞HTR-8/SVneo增殖和侵袭的影响,并探讨其可能的作用机制。方法将HTR-8/Svneo细胞分成A、B、C组,A组加入1 m L/L DMSO,B组加入5×10-8g/m L的BDNF,C组加入0.1μmol/L的BDNF络氨酸激酶受体Trk B特异性抑制剂K252a,24 h后转板继续培养。采用CCK8法观察3组细胞继续培养0、24、48及72 h时的细胞增殖能力(以OD450表示),采用Transwell实验观察3组细胞继续培养24 h时的侵袭能力(以穿膜细胞数表示),采用Western blotting法检测3组细胞继续培养24 h时磷酸化Trk B(p-Trk B)和PI3K/Akt/m TOR信号通路相关蛋白磷酸化蛋白激酶B(p-Akt)、磷酸化雷帕霉素靶蛋白(pm TOR)。结果继续培养48、72 h时B组细胞OD450均高于A组(P均0.05),C组细胞OD450均低于A组(P均0.05)。A、B、C组穿膜细胞数分别为(42±4)、(87±6)、(29±3)个。B组细胞穿膜细胞数多于A组(P0.05),C组细胞穿膜细胞数少于A组(P均0.05)。与A组相比,B组细胞p-Trk B、p-Akt、p-m TOR蛋白表达量均升高(P均0.05),C细胞p-Trk B、p-Akt、p-m TOR表达量均降低(P均0.05)。结论 BDNF可以增强人滋养层细胞HTR-8/Svneo增殖和侵袭能力,其机制可能与其活化PI3K/Akt/m TOR信号通路有关。
[Abstract]:Objective to investigate the effects of brain-derived neurotrophic factor (BDNF) on the proliferation and invasion of human trophoblast cell line (HTR-8/SVneo). Methods HTR-8/Svneo cells were divided into group A and group A with 1 mL / L DMSO. Group B added 5 脳 10 ~ (-8) g / mL BDNFN C group with 0.1 渭 mol/L BDNF complex kinase receptor Trk B specific inhibitor K252a. After 24 h, the cell proliferation ability (expressed as OD450) was observed by CCK8 method. Transwell assay was used to observe the invasiveness (expressed by the number of transmembrane cells) of the three groups after 24 h culture. Western blotting assay was used to detect phosphorylation of Trk Bp-Trk B at 24 h after culture in three groups of cells. And PI3K/Akt/m TOR signaling pathway associated protein phosphorylated protein kinase (BPK). Results the OD450 of group B was higher than that of group A (P 0.05). The OD450 of group C was lower than that of group A (0.05). The number of perforating cells in group C was 42 卤4 (87 卤6). The number of perforating cells in group B was more than that in group A (P 0.05). The number of perforating cells in group C was less than that in group A (P < 0.05), and that in group B was lower than that in group A (P < 0.05). The expression of p-Aktna-p-m TOR protein was significantly increased in both P and P cells, and the expression of p-Trk Bon p-Akt was found in all of the cells. The expression of p-m TOR decreased significantly (P < 0.05). Conclusion BDNF can enhance the proliferation and invasion of HTR-8/Svneo in human trophoblastic cells. The mechanism may be related to the activation of PI3K/Akt/m TOR signaling pathway.
【作者单位】：承德医学院附属医院;献县人民医院;宽城县医院;
【分类号】：R714
【正文快照】： 3宽城县医院)妊娠期间胎盘的成功形成和维持依靠对滋养层细胞增殖和侵袭能力的严格调控[1]。滋养层细胞能够将胎儿和母体组织分隔开并充当两者物质交换的通道,为胎盘提供物理支撑的同时可保护胎儿免受母体免疫排斥反应的伤害[2,3]。胎盘滋养层细胞的功能异常会引起多种妊娠疾

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