梯度密度离心中离心力、时间和梯度液体积对精子回收率和受精潜能的影响

发布时间：2018-01-19 06:24

本文关键词： 离心法梯密度精子受精　出处：《中国男科学杂志》2016年08期 　论文类型：期刊论文

【摘要】：目的探究梯度密度离心中离心力、时间和梯度液体积对前向运动精子回收率和受精潜能的影响。方法实验分新鲜精液组和冷冻精液组各30例。每例精液充分混匀后,平均分为4份,进行下列各组实验:(1)组1为80%和40%的梯度离心液各0.5m L,300×g,离心20min;(2)组2为80%和40%的梯度离心液各1.0m L,300×g,离心20min;(3)组3为80%和40%的梯度离心液各1.0m L,1 200×g,离心5min;(4)组4为80%和40%的梯度离心液各0.5m L,1 200×g,离心5min。分别检测处理前后每组精液浓度、前向运动精子百分率、正常形态率以及精子DNA碎片率,以分析离心力、时间和梯度液体积对精子前向运动精子回收率和受精潜能的影响。结果无论是新鲜精液,还是冷冻精液,组1、组3和组4前向运动精子的回收率和精子的正常形态率以及精子的DNA碎片率与组2比较无明显差异(P0.05)。结论将现行的300×g,离心20min的离心方法,改为1 200×g,离心5min,梯度离心液体积从1.0mL降低至0.5mL,并不显著影响精子正常形态率、前向运动精子的回收率和精子的受精潜能,是可行的。
[Abstract]:Objective to explore the centrifugal force of gradient density. Effects of time and gradient liquid volume on the recovery rate and fertilization potential of forward motile spermatozoa. Methods 30 cases of fresh semen group and 30 cases of frozen semen group were divided into 4 parts on average after each case was fully mixed. The following experiments were carried out in each group: group 1: 80% and 40% gradient centrifugation solution, 0.5 mL / L, 300 脳 g, centrifugation for 20 min; Group 2: 80% and 40% gradient centrifuge solution, 1.0 mL / g, respectively, for 20 min; The gradient centrifuge solution of 80% and 40% in group 3 was 1.0 mL / g each for 5 mins. Group 4: 80% and 40% gradient centrifugals (0.5 mL / g, 1 200 脳 g, respectively) were centrifuged for 5 min. The semen concentration and the percentage of forward motile sperm in each group were measured before and after treatment. Normal morphology rate and sperm DNA fragment rate were used to analyze the effects of centrifugal force, time and gradient liquid volume on the recovery rate and fertilization potential of forward motile spermatozoa. The results were as follows: fresh semen and frozen semen. There was no significant difference between group 1, group 3 and group 4 in the recovery of forward motile sperm, the normal morphology rate of sperm and the rate of DNA fragment in sperm compared with group 2 (P 0.05). The centrifugation method for 20 min was changed to 1 200 脳 g and centrifuged for 5 min. The volume of gradient centrifuge decreased from 1.0 mL to 0.5 mL, and the normal morphology rate of sperm was not significantly affected. The recovery rate of forward motile sperm and the fertilization potential of spermatozoa are feasible.
【作者单位】：国家卫生计生委男性生殖与遗传重点实验室广东省计划生育科学技术研究所生殖中心;
【基金】：广东省计划生育委员会研究项目(编号:20132010) 广东省医学科研基金项目(编号:A2014195)资助
【分类号】：R446.19;R714.8
【正文快照】： 在辅助生育技术中,技术人员要通过相应的精浓度、精子活动率等精液常规分析。子分离技术除去不活动的、形态不正常、不成熟的精(四)用Diffquik染色法进行精子形态学分析子以及精浆,获得高质量的精子进行体外受精。精子依照世界卫生组织人类精液检查与处理实验室分离技术的效率

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