半乳糖凝集素-3和基质金属蛋白酶-9在宫颈癌组织中的表达及其临床意义

发布时间：2018-01-20 09:05

本文关键词： 宫颈癌 Galectin-蛋白 MMP-蛋白免疫组织化学　出处：《中华全科医学》2016年12期 　论文类型：期刊论文

【摘要】：目的探讨半乳糖凝集素-3(Galectin-3)和基质金属蛋白酶-9(MMP-9)在宫颈癌组织中的表达及临床意义。方法用免疫组化SP法检测15例正常宫颈组织、15例宫颈上皮内瘤变组织、48例宫颈癌组织中Galectin-3和MMP-9蛋白的表达情况,并结合临床病理因素进行分析。结果 Galectin-3蛋白在宫颈癌组织中的阳性表达率明显高于正常宫颈组织及宫颈上皮内瘤变组织,MMP-9蛋白在宫颈癌组织中的阳性表达率也明显高于正常宫颈组织和宫颈上皮内瘤变组织,差异均有统计学意义(P0.01)。在宫颈癌组织中,Galectin-3蛋白阳性表达率随病理分级的升高而逐渐升高,有淋巴结转移者高于无淋巴结转移者;同时MMP-9蛋白阳性表达率随病理分级的升高而升高,有淋巴结转移者大于无淋巴结转移者,差异均有统计学意义(P0.05)。在宫颈癌组织中,Galectin-3蛋白和MMP-9蛋白表达呈正相关(P0.01)。结论应用免疫组化的方法检测Galectin-3和MMP-9在宫颈癌中的表达及其临床意义、相关性研究,探讨其在宫颈癌发生发展中的意义,并对宫颈癌治疗提供新的思路。MMP-9蛋白和Galectin-3蛋白的异常表达对宫颈癌细胞恶性转化、增殖和浸润转移有一定的生物学意义,它们之间的这种正反馈效应可能在宫颈癌的发生发展过程中发挥重要的作用,至于其在宫颈癌中发生发展过程中的作用机制需今后进一步研究。
[Abstract]:Objective to investigate galactose-3 Galectin-3) and matrix metalloproteinase-9 (MMP-9). Methods Immunohistochemical SP method was used to detect 15 cases of normal cervical tissues. Expression of Galectin-3 and MMP-9 protein in 48 cases of cervical carcinoma with cervical intraepithelial neoplasia. Results the positive expression rate of Galectin-3 protein in cervical cancer tissues was significantly higher than that in normal cervical tissues and cervical intraepithelial neoplasia tissues. The positive expression rate of MMP-9 protein in cervical cancer tissues was significantly higher than that in normal cervical tissues and cervical intraepithelial neoplasia tissues, and the difference was statistically significant in cervical cancer tissues. The positive expression rate of Galectin-3 protein gradually increased with the increase of pathological grade. The positive expression rate of Galectin-3 protein in patients with lymph node metastasis was higher than that without lymph node metastasis. At the same time, the positive expression rate of MMP-9 protein increased with the increase of pathological grade. The positive expression rate of MMP-9 protein in patients with lymph node metastasis was higher than that without lymph node metastasis, and the difference was statistically significant in cervical cancer tissues. There was a positive correlation between the expression of Galectin-3 protein and MMP-9 protein (P0.01). Conclusion Immunohistochemical method is used to detect the expression of Galectin-3 and MMP-9 in cervical carcinoma and its clinical significance. To explore its significance in the occurrence and development of cervical cancer. The abnormal expression of MMP-9 protein and Galectin-3 protein has certain biological significance for malignant transformation, proliferation, invasion and metastasis of cervical cancer cells. The positive feedback effect between them may play an important role in the occurrence and development of cervical cancer, and the mechanism of its role in the development of cervical cancer needs to be further studied in the future.
【作者单位】：蚌埠医学院第二附属医院妇产科;蚌埠医学院第一附属医院妇产科;
【基金】：安徽省高校自然科学研究项目(KJ2016A482) 安徽省教育厅自然科学研究计划项目(2003kj269)
【分类号】：R737.33
【正文快照】： 宫颈癌是发展中国家女性中最常见的生殖系统恶性肿瘤,与发达国家比较,发展中国家宫颈癌的病死率是发达国家的10倍,并且每年全世界新发病例近50万例,其中中国发病近13万例,占全球发病率的28.8%。然而近年来宫颈癌患者出现了年轻化的现象。其原因可能与性生活开始年龄提前、年轻

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