YB-1在子宫内膜异位症中的表达及意义

发布时间：2018-01-21 14:24

  本文关键词： YB-1 子宫内膜异位症 血清 腹腔液 免疫组化 ELISA　出处：《中南大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的： 通过检测YB-1在子宫内膜异位症组织、腹腔液、血清中的表达情况,探讨YB-1在子宫内膜异位症中的发病机制,探讨YB-1是否可能成为内异症患者一个潜在的诊断指标、治疗靶点及预后标志物。 方法： 应用免疫组化(IHC)SP法检测经病理证实的40例子宫内膜异位症患者中异位内膜、在位内膜及对照组30例正常内膜中YB-1的表达情况。采用ELISA检测YB-1蛋白在40例子宫内膜异位症患者及30例对照组患者血清和腹腔液中的表达变化。探讨YB-1与子宫内膜异位症的关系,并用SPSS17.0软件对其结果进行分析,p0.05具有统计学意义。 结果： (1)YB-1蛋白在正常子宫内膜、在位子宫内膜、异位子宫内膜中的表达呈逐渐增高趋势(P0.05),差异有显著性,主要表达于子宫内膜腺上皮细胞。间质细胞中也有少量表达； (2)内异症组YB-1的水平,无论在血清中还是腹腔液中,均高于对照组(P0.05),且腹腔液中的水平高于血清(P0.05)； (3)在组织中,YB-1的表达在在位内膜中分泌期增生期(P0.05),在异位组和正常内膜组无差异(P0.05)；而在血清和腹腔液中,无论内异症组还是正常组,YB-1在分泌期均高于增生期(P0.05),差异有统计学意义； (4)YB-1在不同R-AFS分期内异症的子宫内膜组织、血清、腹腔液中的表达水平均不同,Ⅲ-Ⅳ期高于Ⅰ-Ⅱ期,差异有统计学意义(P0.05)。 结论： YB-1在子宫内膜异位症组织、血清及腹腔液中高表达,提示YB-1可能参与内异症的发病,并与内异症病变严重程度相关。
[Abstract]:Objective: To investigate the pathogenesis of YB-1 in endometriosis by detecting the expression of YB-1 in endometriosis tissue, peritoneal fluid and serum. To explore the possibility of YB-1 as a potential diagnostic marker, therapeutic target and prognostic marker in patients with endometriosis. Methods: The ectopic endometrium in 40 cases of endometriosis confirmed by pathology was detected by immunohistochemical staining (IHC-SP). The expression of YB-1 in eutopic endometrium and 30 normal endometrium was detected by ELISA in serum and peritoneal fluid of 40 patients with endometriosis and 30 patients with normal endometriosis. To investigate the relationship between YB-1 and endometriosis. The results were analyzed by SPSS17.0 software. Results: The expression of YB-1 protein in normal endometrium, eutopic endometrium and ectopic endometrium increased gradually (P 0.05). Mainly expressed in endometrial glandular epithelial cells. (2) the level of YB-1 in the patients with endometriosis was higher than that in the control group (P 0.05), and the level in the peritoneal fluid was higher than that in the control group (P 0.05). (3) the expression of YB-1 in the eutopic endometrium was in the secretory phase of proliferative phase (P0.05), but there was no difference between the ectopic group and the normal group (P0.05). In both serum and peritoneal fluid, the level of YB-1 in the secretory phase was higher than that in the proliferative phase (P 0.05), both in the endometriosis group and the normal group, and the difference was statistically significant. The expression levels of YB-1 in endometrium, serum and peritoneal fluid of different R-AFS stages were different, and stage 鈪，

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