肝X受体α、胆固醇调节元件结合蛋白1c介导脂质代谢紊乱在子痫前期中的作用及机制

发布时间：2018-01-24 04:44

本文关键词： 子痫前期肝X受体α 胆固醇调节元件结合蛋白c 脂类代谢内皮血管　出处：《中华高血压杂志》2015年02期 　论文类型：期刊论文

【摘要】：目的研究子痫前期患者血清和胎盘中肝X受体α(LXRα)及其靶基因胆固醇调节元件结合蛋白1c(SREBP-1c)的表达与脂质代谢紊乱的关系及机制。方法入选子痫前期患者32例(轻度子痫前期15例和重度子痫前期17例)、正常妊娠30例和正常未孕30例。测定各组血脂水平,计算动脉硬化指数[动脉硬化指数=(总胆固醇-高密度脂蛋白胆固醇)/高密度脂蛋白胆固醇];酶联免疫吸附试验(ELISA)测定各组血清中LXRα及SREBP-1c蛋白水平;逆转录-聚合酶链反应(RT-PCR)及免疫组化技术(IHC)检测胎盘中氧化型低密度脂蛋白(oxLDL)、LXRα及SREBP-1c的mRNA和(或)蛋白表达水平。结果子痫前期组与正常妊娠组比较,总胆固醇[(6.78±1.56)比(5.20±0.96)mmol/L]、三酰甘油[(3.92±1.35)比(2.48±0.78)mmol/L]、极低密度脂蛋白胆固醇[(1.85±0.53)比(1.12±0.35)mmol/L]、低密度脂蛋白胆固醇[(4.31±1.23)比(3.01±0.55)mmol/L]、动脉硬化指数(3.10±1.22比1.88±0.74)增高,高密度脂蛋白胆固醇降低(P0.01或P0.05);正常妊娠组,轻、重度子痫前期组血清及胎盘中oxLDL、LXRα、SREBP-1c的mRNA和(或)蛋白的表达逐步升高(P0.01或P0.05)。相关分析显示,血清LXRα蛋白与血清SREBP-1c蛋白、总胆固醇、三酰甘油、动脉硬化指数呈正相关,与高密度脂蛋白胆固醇呈负相关。结论子痫前期患者血清及胎盘LXRα表达明显增强,促进其靶基因SREBP-1c的转录和翻译,介导母体及胎盘的脂质代谢紊乱。
[Abstract]:Objective to study the liver X receptor alpha in serum and placenta in preeclampsia (LXR alpha) and its target gene of sterol regulatory element binding protein 1c (SREBP-1c) and the relationship between the expression of lipid metabolism and mechanism. 32 cases of patients with preeclampsia were selected method (15 cases of mild preeclampsia and severe preeclampsia, 30 cases and 17 cases) normal non pregnant 30 cases of normal pregnancy. The determination of the level of serum cholesterol, arteriosclerosis index calculation [atherosclerosis index = (total cholesterol and high density lipoprotein cholesterol) / high density lipoprotein cholesterol]; enzyme linked immunosorbent assay (ELISA) determination of the serum protein level of LXR alpha and SREBP-1c; reverse transcriptase polymerase chain the reaction (RT-PCR) and immunohistochemistry (IHC) assay in placenta of oxidized low density lipoprotein (oxLDL), LXR alpha and SREBP-1c mRNA and (or) protein expression level in preeclampsia group. Results compared with the normal pregnancy group, the total cholesterol ([6.78 + 1.56) than (5.20 + 0.96) mmol/L], three glycerol [(3.92 + 1.35) than mmol/L] (2.48 + 0.78), very low density lipoprotein cholesterol [(1.85 + 0.53) than mmol/L] (1.12 + 0.35), low density lipoprotein cholesterol [(4.31 + 1.23) than (3.01 + 0.55 mmol/L]), arterial stiffness index (3.10 + 1.22 to 1.88 + 0.74) increased high density lipoprotein cholesterol (P0.01 or P0.05); normal pregnancy group, severe preeclampsia group, oxLDL in serum and placenta of LXR alpha, SREBP-1c mRNA and (or) protein expression gradually increased (P0.01 or P0.05). Correlation analysis showed that serum LXR protein and serum SREBP-1c protein, total cholesterol, triglyceride was three, atherosclerosis index correlated negatively correlated with high density lipoprotein cholesterol in patients with preeclampsia. Conclusion serum and placental LXR expression increased significantly, promote its target gene SREBP-1c transcription and translation, lipid metabolism mediated by maternal and placental Disorder.

【作者单位】：福建中医药大学附属第二人民医院妇产科;福建医科大学附属第一医院妇产科;
【基金】：福建医科大学教授学术发展基金(JS08012)
【分类号】：R714.244
【正文快照】： 子痫前期是孕产妇死亡的主要原因之一。其病因及机制的学说众多,各学说之间互相关联、互相影响。目前较为公认的是内皮细胞激活和损伤学说。内皮细胞损伤是子痫前期发生的中心环节。近年研究发现肝X受体α(liver X receptorα,LXRα)与脂类代谢、血管内皮损伤、滋养细胞增殖和

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