内皮型一氧化氮合酶基因G894T多态性与青海省汉族妊娠高血压综合征的相关性

发布时间：2018-01-25 05:29

  本文关键词： 妊娠高血压综合征 多态性 内皮型一氧化氮合酶 限制性片段长度多态性聚合酶链反应 孕妇　出处：《解剖学报》2017年03期 　论文类型：期刊论文

【摘要】：目的探讨内皮型一氧化氮合酶(eNOS)G894T基因多态性与青海省汉族妊娠高血压综合征(PIH)的相关性。方法采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)方法对138名妊娠高血压综合征患者和135名正常孕妇e NOS基因G894T多态性进行分型,并测序验证。结果妊娠高血压综合征组基因型频率野生型(GG)、杂合子型(GT)和突变纯合子型(TT)分别为17.4%、82.6%、0;对照组分别为0、95.6%、4.4%,两组基因型频率分布有差异(P0.05)。妊娠高血压综合征组e NOS等位基因频率G、T分别为58.7%、41.3%;对照组分别为47.8%、52.2%,两组比较差异有统计学意义(P0.05),妊娠高血压综合征组G基因频率高于对照组。结论e NOS基因G894T多态性可能与青海省汉族妊娠高血压综合征有关,G等位基因可能为妊娠高血压综合征的易感基因(OR=1.229,95%CI:1.048~1.441),T等位基因可能为妊娠高血压综合征的保护基因。携带GG基因型的孕妇可作为青海省妊娠高血压综合征的易感人群。
[Abstract]:Objective to investigate the relationship between the polymorphisms of endothelial nitric oxide synthase (Enos) G894T gene and PIH in the Han nationality of Qinghai Province. Methods restriction fragment length polymorphism polymerase chain reaction (PCR RFLP) was used. Methods the polymorphisms of e NOS gene G894T in 138 patients with pregnancy-induced hypertension and 135 normal pregnant women were classified. Results the genotypes of GGG, GTT and TTT were 17.4% and 82.6% respectively in patients with pregnancy-induced hypertension syndrome (PIH). The genotype frequencies of the two groups were significantly different (P 0.05). The allele frequency of e NOS was G in the pregnancy induced hypertension syndrome group. T was 58.7 and 41.3 respectively; The control group was 47.8% and 52.2% respectively. The difference between the two groups was statistically significant (P 0.05). Conclusion the G894T polymorphism of e NOS gene may be associated with pregnancy-induced hypertension syndrome of Han nationality in Qinghai province. G allele may be the susceptibility gene of pregnancy induced hypertension syndrome. The T allele may be a protective gene for pregnancy-induced hypertension syndrome (PIH) and pregnant women with GG genotype can be used as susceptible populations of PIH in Qinghai province.
【作者单位】： 青海大学医学院基础医学部解剖学教研室;
【基金】：青海省科技厅计划项目(2015-ZJ-746)
【分类号】：R714.246
【正文快照】： 妊娠高血压综合征(pregnancy-inducedhypertension syndrom,PIH)是产科常见疾病,是引起孕产妇及胎儿死亡的主要原因。国外发病率为7%~12%,我国为9.4%,高原地区为24%左右[1]。一氧化氮(NO)是重要的血管紧张性调节分子,在卵泡发育和成熟,胚胎植入,妊娠维持,分娩等许多生理过程中，

本文编号：1462119