上皮性卵巢癌组织CK2β和p53蛋白表达临床意义分析

发布时间：2018-01-25 04:51

本文关键词： 卵巢肿瘤 CKβ p 免疫组织化学预后　出处：《中华肿瘤防治杂志》2015年06期 　论文类型：期刊论文

【摘要】：目的研究蛋白激酶CK2β及抑癌基因蛋白p53在卵巢癌组织中的表达情况,并分析其表达与卵巢癌临床病理参数及预后的关系。方法收集2003-01-01-2005-12-31就诊于浙江大学医学院附属妇产科医院的上皮性卵巢肿瘤患者148例(包括108例上皮性卵巢癌,20例卵巢良性上皮性肿瘤及20例卵巢交界性肿瘤),应用免疫组化法检测CK2β及p53在肿瘤组织中的表达情况,并分析两者在不同肿瘤组织中的表达差异及其与上皮性卵巢癌预后的关系。结果上皮性卵巢癌组织CK2β阳性表达68例(63.0%),p53阳性表达58例(53.7%);卵巢交界性肿瘤CK2β阳性表达6例(30.0%),p53阳性表达4例(20.0%);卵巢良性上皮性肿瘤CK2β和p53阳性表达均为0例;CK2β在卵巢癌组织中的表达强度显著高于卵巢良性肿瘤组织(P0.001)和交界性肿瘤组织(P=0.003);p53在卵巢癌组织中的表达强度显著高于卵巢良性肿瘤组织(P0.001)及交界性肿瘤组织(P=0.006);CK2β和p53之间具有显著相关性,r=0.377,P0.001。CK2β蛋白表达与卵巢癌临床分期(P=0.001)、术前血清CA125水平(P0.001)及化疗敏感性(P=0.012)显著相关;p53蛋白表达与组织分化程度(P=0.027)及术前血清CA125水平(P=0.027)相关。CK2β及p53阳性表达与上皮性卵巢癌患者的无瘤生存预后及总生存预后显著相关(P值均0.05);多因素回归显示,临床分期(P0.001)和p53阳性表达(P=0.018)可作为预测卵巢癌无瘤生存预后的独立因素,而临床分期(P=0.001)、术前CA125水平(P=0.040)和p53阳性表达(P=0.041)可作为预测卵巢癌总生存预后的独立因素。结论 CK2β和p53蛋白阳性表达可能与卵巢癌的发生发展有密切关系,p53阳性表达对卵巢癌预后预测具有一定的价值。
[Abstract]:Objective to study the expression of protein kinase CK2 尾 and tumor suppressor gene p53 in ovarian carcinoma. The relationship between the expression and clinicopathological parameters and prognosis of ovarian cancer was analyzed. Methods the epithelial oocytes were collected from the affiliated Obstetrics and Gynecology Hospital of Zhejiang University from January 2003 to January 2005. 148 cases of Nest tumor. Including 108 cases of epithelial ovarian cancer. The expressions of CK2 尾 and p53 in 20 cases of benign epithelial ovarian tumors and 20 cases of ovarian borderline tumors were detected by immunohistochemical method. The relationship between the expression of CK2 尾 and the prognosis of epithelial ovarian cancer was analyzed. Results 68 cases of epithelial ovarian carcinoma were positive for CK2 尾 expression. The positive expression of p53 was 53.7% in 58 cases. The positive expression of CK2 尾 in 6 cases of ovarian borderline tumor was 30.0%, and the positive expression of p53 in 4 cases was 20.0%. Positive expression of CK2 尾 and p53 was found in 0 cases of benign epithelial ovarian tumors. The expression of CK2 尾 in ovarian carcinoma was significantly higher than that in benign ovarian tumor (P0.001) and borderline tumor (P0.003). The expression of p53 in ovarian carcinoma was significantly higher than that in benign ovarian tumor (P0.001) and borderline tumor (P0. 006). There was a significant correlation between the expression of CK2 尾 and p53 protein and the clinical stage of ovarian cancer (P 0.001). There was a significant correlation between preoperative serum CA125 level (P 0.001) and chemotherapeutic sensitivity (P < 0.012). Expression of p53 protein and degree of tissue differentiation P0. 027) and preoperative serum CA125 level P0. 027). The positive expression of CK2 尾 and p53 was significantly correlated with the survival and survival of epithelial ovarian cancer patients (P < 0.05). Multivariate regression analysis showed that the clinical staging (P0.001) and p53 positive expression (P0.018) could be used as independent factors to predict the survival and prognosis of ovarian cancer without tumor. The clinical stage was P0. 001). Preoperative CA125 level and p53 positive expression (P0. 040) and p53 positive expression (P0. 041). Conclusion the positive expression of CK2 尾 and p53 protein may be closely related to the occurrence and development of ovarian cancer. P53 positive expression has a certain value in predicting the prognosis of ovarian cancer.
【作者单位】：湖州市中心医院妇产科;浙江大学医学院附属妇产科医院肿瘤科;浙江大学医学院附属妇产科医院病理科;
【基金】：国家高技术研究发展计划(863计划,2012AA02A507)
【分类号】：R737.31
【正文快照】： 中华肿瘤防治杂志,2015,22(6):451-456Chin J Cancer Prev Treat,2015,22(6):451-456卵巢癌发病隐匿,侵袭性强,目前,其发病率呈逐年上升趋势,死亡率居高不下,已成为严重威胁女性健康的恶性肿瘤之一[1]。造成卵巢癌高死亡率的原因主要为卵巢癌的早期症状不明显,其恶性程度高,远

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