IGF1R在卵巢子宫内膜异位囊肿中的表达及意义

发布时间：2018-01-25 07:02

本文关键词： IGFR 子宫内膜异位症反义寡核苷酸细胞增殖　出处：《现代妇产科进展》2017年08期 　论文类型：期刊论文

【摘要】：目的:检测IGF1R在正常子宫内膜及卵巢子宫内膜异位囊肿(EMs)患者异位内膜中的表达,并探讨其与疾病发生的关系。方法:免疫组化SP染色及Western blot法检测IGF1R在50例正常子宫内膜及55例卵巢EMs异位内膜中的表达情况,并根据月经周期对样本进行分期分析。构建IGF1R的反义寡核苷酸,转染至正常内膜的原代细胞,运用Ed U技术研究IGF1R对细胞增殖的影响。结果:免疫组化检测显示,IGF1R表达于正常内膜及EMs组织的腺上皮细胞质及胞膜,异位内膜中表达量较高(P=0.014)。增生期正常内膜及异位内膜中IGF1R的表达差异无统计学意义;分泌期异位内膜中IGF1R的表达量高于分泌期正常子宫内膜(P=0.036)。分泌期子宫内膜中IGF1R表达显著高于增生期子宫内膜,差异有统计学意义(P0.001)。Western blot检测结果显示,异位内膜中IGF1R表达量较正常内膜高(P0.05);增生期正常内膜和异位内膜中IGF1R表达量差异无统计学意义;分泌期正常子宫内膜和异位内膜中IGF1R表达量分别为(0.26±0.13)和(0.65±0.16),差异有统计学意义(P=0.023)。正常内膜的原代细胞中沉默IGF1R表达后,细胞增殖能力明显下降(P=0.014)。结论:IGF1R在异位内膜中的表达量较正常内膜高,并且参与异位内膜的细胞增殖,提示IGF1R可能通过促进异位内膜细胞的增殖参与EMs的发生发展。
[Abstract]:Objective: to detect the expression of IGF1R in ectopic endometrium of normal endometrium and ovarian endometriosis cysts. Methods: immunohistochemical SP staining and Western. The expression of IGF1R in 50 cases of normal endometrium and 55 cases of ovarian EMs ectopic endometrium was detected by blot method. The antisense oligodeoxynucleotides of IGF1R were constructed and transfected into the primary cells of normal endometrium according to the menstrual cycle. Results: the expression of IGF1R in glandular epithelial cytoplasm and membrane of normal endometrium and EMs tissue was detected by immunohistochemistry. The expression of IGF1R in ectopic endometrium was higher than that in normal endometrium and ectopic endometrium. There was no significant difference in the expression of IGF1R between normal and ectopic endometrium. The expression of IGF1R in ectopic endometrium during secretory phase was higher than that in normal endometrium during secretory phase, and the expression of IGF1R in secretory endometrium was significantly higher than that in proliferative endometrium. The expression of IGF1R in ectopic endometrium was higher than that in normal endometrium (P 0.05). There was no significant difference in the expression of IGF1R between normal and ectopic endometrium in proliferative phase. The expression of IGF1R in normal endometrium and ectopic endometrium was 0.26 卤0.13) and 0.65 卤0.16 respectively. The difference was statistically significant (P < 0. 023). After silencing IGF1R expression in primary cells of normal endometrium. Conclusion the expression of 1% IGF1R in ectopic endometrium is higher than that in normal endometrium, and it is involved in the proliferation of ectopic endometrium. The results suggest that IGF1R may play a role in the development of EMs by promoting the proliferation of ectopic endometrial cells.
【作者单位】：华中科技大学同济医学院附属武汉市中心医院妇产科;贵州医科大学附属医院妇产科;华中科技大学同济医学院附属同济医院妇产科;
【基金】：中央高校基本科研业务费专项资金资助(No:2016YXMS115)
【分类号】：R711.71
【正文快照】： 子宫内膜异位症(Endometriosis,EMs)是生殖期妇女最常见的妇科疾病之一,其发病率为10%~15%[1],主要引起痛经、慢性盆腔痛及不孕。目前临床常将盆腔内异症主要分为3型:腹膜型、卵巢型和深部浸润型,卵巢型最常见[2]。EMs为良性疾病,却表现出侵袭、血管形成、复发等恶性行为。较

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