芳香烃受体在宫颈上皮内瘤变和宫颈癌组织中的表达与意义

发布时间：2018-01-25 13:59

  本文关键词： 宫颈癌 宫颈上皮内瘤样变 芳香烃受体　出处：《上海医学》2015年09期 　论文类型：期刊论文

【摘要】：目的通过观察宫颈上皮内瘤变(CIN)和宫颈癌患者外周血、局部组织中芳香烃受体(AhR)的表达,探讨AhR与CIN和宫颈癌的关系,为开拓CIN和宫颈癌的治疗新途径提供理论和实验依据。方法选取2010年6月1日-2013年6月1日上海市浦东新区人民医院和上海交通大学医学院附属仁济医院的门诊和住院患者共140例,因子宫肌瘤、子宫肌腺症、功能障碍性子宫出血等各种子宫良性病变而行子宫切除,经病理学检查证实,其中宫颈组织正常者30例(对照组)、CIN患者65例(其中CINⅡ39例和CINⅢ26例,CIN组)、宫颈癌患者45例(宫颈癌组),采用免疫组织化学和Western印迹法检测各组患者局部组织中AhR的表达。结果免疫组织化学检测结果显示,3组患者局部组织中的AhR阳性信号均位于细胞质和细胞核。宫颈癌组的AhR指数为85.56±5.79,显著高于CIN组的57.25±4.71(t=28.18,P0.05);CIN组的AhR指数显著高于对照组的2.29±0.52(t=63.48,P0.05)。Western印迹法检测结果显示,各组局部组织中均有AhR蛋白表达,随着CIN向宫颈癌的进展,AhR蛋白表达量逐渐增多。宫颈癌组的AhR蛋白相对灰度值为74.96±6.28,显著高于CIN组的58.33±7.65(t=12.04,P0.05);CIN组的AhR蛋白相对灰度值显著高于对照组的14.40±6.00(t=27.75,P0.05)。结论 AhR参与了CIN和宫颈癌的发生、发展,AhR可能为CIN和宫颈癌的治疗提供新的靶点。
[Abstract]:Objective to investigate the relationship between AhR and CIN and cervical cancer by observing the expression of AhR in peripheral blood and local tissues of cervical cancer patients with cervical intraepithelial neoplasia (cin). To provide theoretical and experimental evidence for the development of new approaches to the treatment of CIN and cervical cancer. Methods from June 1st 2010 to June 1st 2013, Shanghai Pudong New District people's Hospital and Shanghai Jiaotong University School of Medicine were selected. There were 140 outpatients and inpatients in the affiliated Yan Chai Hospital. Hysterectomy was performed for uterine leiomyoma, myometriosis, dysfunctional uterine bleeding and other benign uterine lesions. 30 cases of normal cervical tissue were confirmed by pathological examination (control group). 65 CIN patients (39 CIN 鈪，

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