miR-210与子痫前期滋养层细胞自噬的关系

发布时间：2018-01-25 23:21

  本文关键词： 子痫前期 miR- 自噬　出处：《广东医学》2017年13期 　论文类型：期刊论文

【摘要】：目的探讨子痫前期胎盘组织中miR-210和自噬情况,并初步阐明其可能的分子机制。方法收集子痫前期患者(子痫前期组)和同期住院正常晚期妊娠孕妇(正常对照组)各20例,应用Real-time PCR检测胎盘组织中miR-210和自噬标志基因Beclin-1、LC3Ⅱ的表达水平,分离培养胎盘滋养层细胞,采用miR-210抑制剂(anti-miR-210)敲低miR-210,通过real-time PCR检测Beclin-1、LC3Ⅱ的表达变化情况。结果子痫前期组胎盘组织内miR-210表达增加,自噬增多,Beclin-1和LC3Ⅱ表达量较正常对照组明显升高,敲低miR-210后显著抑制自噬反应,Beclin-1和LC3Ⅱ的表达下降,表明二者之间存在协同关系。结论 miR-210参与调控子痫前期滋养层细胞自噬。
[Abstract]:Objective to investigate miR-210 and autophagy in placenta of preeclampsia. Methods Twenty patients with preeclampsia (pre-eclampsia group) and 20 pregnant women with normal late pregnancy (normal control group) were collected. Real-time PCR was used to detect the expression of miR-210 and the autophagy marker gene Beclin-1mLC3 鈪，

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