乏氧条件下HIF-1α介导Hela宫颈癌细胞的放射敏感性

发布时间：2018-01-26 01:18

本文关键词： 缺氧电离辐射低氧诱导因子-α 血管内皮生成因子宫颈癌 p　出处：《现代肿瘤医学》2016年03期 　论文类型：期刊论文

【摘要】：目的:探讨乏氧条件下,乏氧诱导因子HIF-1α对Hela宫颈癌细胞放射敏感性的影响以及初步作用机制。方法:MTT法分析不同组别宫颈癌细胞存活分数,流式细胞法(FCM)检测各组细胞的凋亡变化,RT-PCR和Western blot分别检验各组宫颈癌细胞的HIF-1α、VEGF和p53的mRNA和蛋白表达情况,siRNA干扰HIF-1α对宫颈癌放射敏感性的影响,以及对VEGF、p53表达的影响。结果:乏氧条件下通过诱导肿瘤细胞HIF-1α的高表达途径,提高VEGF表达,抑制p53表达来降低Hela宫颈癌细胞的放射敏感性。HIF-1α的siRNA转染联合照射后,在乏氧条件下MTT法显示细胞存活率较非转染组明显下降,流式细胞仪法显示细胞凋亡率明显上升。同时干扰HIF-1α后明显抑制VEGF的表达,提高p53的表达。结论:抑制HIF-1α可能通过减少VEGF及增加p53来提高Hela宫颈癌细胞的放射敏感性。
[Abstract]:Objective: to explore the condition of hypoxia. Effect of hypoxia inducible factor HIF-1 伪 on radiosensitivity of Hela cervical cancer cells and its mechanism. Apoptosis was detected by flow cytometry (FCM). RT-PCR and Western blot were used to detect HIF-1 伪 of cervical cancer cells in each group. The expression of mRNA and protein in VEGF and p53. The effect of siRNA interference HIF-1 伪 on radiosensitivity of cervical cancer and VEGF. Results: the high expression of HIF-1 伪 in tumor cells was induced by hypoxia, and the expression of VEGF was increased. Inhibition of p53 expression to reduce radiosensitivity of Hela cervical cancer cells. HIF-1 伪 siRNA transfection combined with irradiation. Under hypoxia condition, the survival rate of MTT was significantly lower than that of non-transfection group, and the apoptosis rate was significantly increased by flow cytometry, and the expression of VEGF was inhibited by interfering with HIF-1 伪. Conclusion: inhibition of HIF-1 伪 may increase radiosensitivity of Hela cervical cancer cells by decreasing VEGF and increasing p53.
【作者单位】：福建医科大学福总临床医学院南京军区福州总医院放疗科;福建医科大学福总临床医学院南京军区福州总医院妇产科;
【基金】：福建省自然科学基金(编号:2014J01432)
【分类号】：R737.33
【正文快照】： 随着宫颈癌治疗技术的不断提高,宫颈癌的手术及非手术治疗手段有明显的长足进步,但宫颈癌的整体生存率并没有得到显著提高。目前非手术治疗手段包括放射治疗及化疗,它们均能消灭大部分肿瘤增殖细胞,然而,仍有部分宫颈癌细胞不能被这些常规手段所根除。其中肿瘤微环境在宫颈癌

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