阿帕替尼对宫颈癌细胞增殖及化疗敏感性的影响

发布时间：2018-01-26 08:45

本文关键词： 阿帕替尼宫颈癌周期化疗敏感性　出处：《现代妇产科进展》2017年11期 　论文类型：期刊论文

【摘要】：目的:探讨阿帕替尼(Apatinib)对人宫颈癌细胞的抑制增殖作用以及对宫颈癌化疗药物敏感性的影响。方法:CCK-8实验和克隆形成实验分别检测apatinib对宫颈癌细胞Si Ha和HCC94的增殖抑制作用,流式细胞术检测apatinib对宫颈癌细胞周期的影响,RT-qPCR检测Cyclin D3 mRNA表达,Western blot检测Cyclin D3蛋白表达。结果:Apatinib呈剂量依赖性抑制宫颈癌细胞的增殖;apatinib可诱导宫颈癌细胞发生G_0/G_1期阻滞,显著降低宫颈癌Si Ha和HCC94细胞中Cyclin D3 mRNA及Cyclin D3蛋白的表达水平(P0.01);apatinib可提高宫颈癌细胞对紫杉醇的化疗敏感性。结论:Apatinib可通过诱导宫颈癌细胞G_0/G_1期阻滞抑制其增殖,且对紫杉醇抑制宫颈癌细胞的生长有增效作用。
[Abstract]:Objective: to study the Apatinib of Apatinib. Inhibition of proliferation and chemosensitivity to chemotherapeutic agents in human cervical cancer cells. Methods apatinib was used to detect the effect of apatinib on Si in cervical cancer cells. Ha and HCC94 inhibited proliferation. The effect of apatinib on the cell cycle of cervical carcinoma was detected by flow cytometry. RT-qPCR was used to detect the expression of Cyclin D3 mRNA. The expression of Cyclin D3 protein was detected by Western blot. Results the proliferation of cervical cancer cells was inhibited in a dose-dependent manner. Apatinib could induce G\ + 0 / G\ -1 arrest in cervical cancer cells. The expression level of Cyclin D3 mRNA and Cyclin D3 protein in Cervical carcinoma SIHA and HCC94 cells was significantly decreased (P 0.01). Apatinib can increase the chemosensitivity of cervical cancer cells to paclitaxel. Conclusion the proliferation of cervical cancer cells can be inhibited by inducing G _ (0) / G _ (1) phase block of cervical cancer cell line G _ (0) / G _ (1). In addition, paclitaxel can inhibit the growth of cervical cancer cells.
【作者单位】：无锡市第四人民医院妇科;江南大学无锡医学院;郑州大学第一附属医院肿瘤科;江苏大学;郑州大学第一附属医院妇科;
【基金】：江苏省无锡市科技局项目(No:CSE31N1418) 无锡市卫生局项目(No:MS201417、FYKY201401) 无锡市医院管理中心科研项目(No:YGZXG1405) 国家自然科学基金资助项目(No:81502262) 河南省博士后科研项目(No:2014021) 河南省医学科技攻关项目(No:201503045)
【分类号】：R737.33
【正文快照】： Effect of apatinib on inhibiting proliferation and increasing chemosensitivity of cervicalcancer cells.Liu Qiuli1,2,Li Jing3,Li Juan4,et al.1.Department of Gynecology,Affiliated Hospi-tal of Jiangnan University,the Fourth People's Hospital of Wuxi City,W

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