怀有先天性心脏病胎儿的孕妇与正常孕妇肠道菌群的高通量测序分析

发布时间：2018-01-31 04:06

本文关键词： 肠道菌群 Illumina测序双歧杆菌科红蝽杆菌科先天性心脏病　出处：《中国病理生理杂志》2015年04期 　论文类型：期刊论文

【摘要】：目的:分析怀有先天性心脏病胎儿的孕妇(PW组)与正常孕妇(NW组)的肠道菌群特征。方法:对可能引起个体肠道菌群差异的因素加以控制,2013年6月18号~2013年12月17号收集15例正常孕妇和17例异常孕妇的粪便样本,提取细菌基因组DNA,用PCR方法扩增16S r DNA,进行二代Illumina测序。测序数据应用运算分类单位(operational taxonomic unit,OTU)聚类分析、多样性分析和分类学分析等生物信息技术分析2类孕妇肠道菌群的结构、多样性和丰度。结果:2类孕妇肠道菌群的高通量测序分析分别得到2 696 276(NW组)条和2 445 530(PW组)条优化序列,测序覆盖深度97%。经过97%相似度归并后分别得到77 243个(NW组)和75 600个OTUs(PW组);菌群多样性分析显示正常孕妇肠道菌群的Chao 1指数和Shannon指数均高于异常孕妇。在最佳分类水平上,正常孕妇肠道菌群由38个科的细菌构成,归属于14个门,以厚壁菌门(Firmicutes)为主,其次为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)等。怀有先心胎儿孕妇肠道菌群构成与正常孕妇相似,最佳分类水平上由33个科的细菌构成,归属于9个门,其中Firmicutes为(75.7±15.8)%,Proteobacteria为(21.1±17.0)%,Actinobacteria为(2.0±2.1)%。在最佳分类水平上,方差分析显示两者的双歧杆菌科(Bifidobacteriaceae)和红蝽杆菌科(Coriobacteriaceae)菌种丰度有明显差异。结论:PW组孕妇与NW组孕妇相比,整体肠道菌群含量明显下降,提示PW组孕妇有更恶劣的肠道环境,其中部分菌群含量存在明显差异。先天性心脏病胎儿的发生可能与孕妇恶劣的肠道微环境有关,其中Bifidobacteriaceae和Coriobacteriaceae在先天性心脏病的预防方面可能有重要作用。
[Abstract]:Objective: to analyze the intestinal flora of pregnant women with congenital heart disease (PW group) and normal pregnant women (NW group). From June 18th 2013 to December 17th 2013, the fecal samples of 15 normal pregnant women and 17 abnormal pregnant women were collected and the bacterial genomic DNA was extracted. 16s r DNA was amplified by PCR. The second generation Illumina sequencing was carried out. The sequence data were analyzed by the operational taxonomic Unit (OTU) cluster analysis. Biological information techniques such as diversity analysis and taxonomic analysis were used to analyze the structure of intestinal flora of two groups of pregnant women. Results the high throughput sequencing analysis of the intestinal microflora of two groups of pregnant women obtained the optimized sequence of 2 696 276NW and 2 445 530 PW groups, respectively. After 97% similarity, 77,243 NW and 75,600 OTUs(PW groups were obtained. The analysis of bacterial diversity showed that the Chao 1 index and Shannon index of normal pregnant women were higher than those of abnormal pregnant women. The intestinal flora of normal pregnant women is composed of 38 families, belonging to 14 phylum, mainly phylum firmicutes. The next is Proteobacteria, Actinobacteria). The composition of intestinal flora of pregnant women with precore fetus is similar to that of normal pregnant women. The best taxonomic level was composed of 33 families of bacteria, belonging to 9 phyla, of which Firmicutes was 75.7 卤15.8%. The Proteobacteria was 21.1 卤17.0 and Actinobacteria was 2.0 卤2.1 at the best classification level. Analysis of variance showed that Bifidobacteriaceaeand Coriobacteriaceaeae of the family Bifidobacteriaceaeand Coriobacteriaceae. Conclusion there is significant difference in the abundance of bacteria between the two groups. Conclusion the proportion of pregnant women in the w w group is higher than that in the NW group. The total intestinal flora decreased significantly, suggesting that PW group had a worse intestinal environment. The occurrence of congenital heart disease fetus may be related to the poor intestinal microenvironment of pregnant women. Bifidobacteriaceae and Coriobacteriaceae may play an important role in the prevention of congenital heart disease.
【作者单位】：广东省心血管病研究所;广东省人民医院广东省医学科学院;南方医科大学研究生学院;
【分类号】：R714.2
【正文快照】： 肠道菌群被誉为是人体自身的黑匣子,一切的生命活动和代谢情况,都可以从中窥见一斑[1]。已证实肠道菌群与人体过敏反应、自身免疫病、肥胖、糖尿病、心血管疾病、自闭症和神经系统疾病等都有明确的关系[2]。考虑到一切的外在因素最终都要通过母体影响胎儿的发育,被誉为人体另

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