宫颈癌中Snail、Slug和KAI1的表达及临床意义

发布时间：2018-02-03 18:23

本文关键词： 宫颈肿瘤 Snail Slug KAI 预后　出处：《南方医科大学学报》2015年12期 　论文类型：期刊论文

【摘要】：目的检测宫颈癌中上皮间充质转化(EMT)相关标记物Snail、Slug和转移抑制因子KAI1的表达,并分析它们的相互关系及其临床病理意义。方法利用免疫组化Elivision TM plus法检测154例宫颈鳞状细胞癌(CSCC)、50例宫颈上皮内瘤变(CIN)和40例正常宫颈上皮组织中Snail、Slug和KAI1蛋白的表达。结果 Snail、Slug和KAI1在CSCC组、CIN组和对照组的阳性表达率分别为66.2%、66.9%、43.5%;32.0%、34.0%、64.0%和0%、2.5%、95.0%,两组相互之间差异有统计学意义(P0.05)。Snail、Slug和KAI1蛋白的表达均与CSCC肿瘤组织的分化程度、淋巴结转移与否、浸润深度、FIGO分期及术后生存期均有关(P0.05);Snail蛋白的表达与Slug蛋白的表达呈正相关关系(r=0.752,P0.001),Snail和Slug蛋白的表达均与KAI1蛋白的表达呈负相关关系(P0.001)。Kaplan-Meier生存分析表明,Snail和Slug蛋白表达阳性的患者其生存率明显低于其阴性表达的患者(P0.001);KAI1蛋白表达阳性的患者其生存率明显高于其阴性表达的患者(P0.001)。Cox回归分析显示Snail和KAI1蛋白的阳性表达及FIGO分期是影响CSCC患者术后生存的独立预后因素。结论 Snail、Slug和KAI1蛋白的表达水平在CSCC组织中与肿瘤组织的分化程度、FIGO、浸润深度、淋巴结转移与否及预后等均有关;在CSCC组织中早期联合检测Snail、Slug和KAI1对判断预后有价值。
[Abstract]:Objective to detect the expression of Slug and KAI1 in epithelial mesenchymal transformation (EMT) related markers in cervical carcinoma. The relationship between them and their clinicopathological significance were analyzed. Methods the protein levels of Slug and KAI1 in 50 cases of cervical squamous cell carcinoma and 40 cases of normal cervical epithelium were detected by immunohistochemical Elivision TM plus method. Results the positive expression rates of Slug and KAI1 in CSCC group were 66.2 and 43.9%, respectively. The expression rates of Slug and KAI1 in CSCC group were 64.0% and 32.0%, respectively. There was significant difference between the two groups in the expression of Slug protein and KAI1 protein in CSCC tumor tissue. Lymph node metastasis, FIGO stage and survival time after operation were positively correlated with the expression of Slug protein. Both the expression of snail protein and Slug protein were negatively correlated with the expression of KAI1 protein. Kaplan-Meier survival analysis showed that snail protein and Slug protein surface were negatively correlated with the expression of KAI1 protein. The survival rate of patients with positive expression was significantly lower than that of patients with positive expression of P0.001KAI1 protein. The survival rate of patients with positive expression of KAI1 protein was significantly higher than that of patients with negative expression of KAI1 protein. Regression analysis showed that the positive expression of Snail and KAI1 protein and FIGO stage were significantly higher than those of patients with negative expression of KAI1 protein. Conclusion the expression of Slug and KAI1 protein in CSCC tissues is different from that in tumor tissues, and the depth of invasion is higher than that of Slug protein, and the expression of Slug protein and KAI1 protein in CSCC tissues are different from those in tumor tissues. Both lymph node metastasis and prognosis were related to the early detection of Snailn Slug and KAI1 in CSCC tissues.
【作者单位】：蚌埠医学院第一附属医院病理科//蚌埠医学院病理教研室;
【基金】：安徽省自然科学基金(1408085MH147)
【分类号】：R737.33
【正文快照】： 尽管近年来,宫颈癌的治疗方法和技术有了很大的提高,但其总的5年生存率没有明显提高,复发和转移是其主要原因。转移是指肿瘤细胞通过自身变形,脱离原发瘤,降解细胞外基质及新生血管形成而完成侵袭及转移过程。上皮间充质转化(EMT)是指上皮细胞在特定的生理和病理情况下向间充

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