顺铂上调STAT1蛋白抑制宫颈癌细胞增殖

发布时间：2018-02-04 00:18

本文关键词： Stat c-Myc 宫颈癌 Hela细胞顺铂化学治疗　出处：《南方医科大学学报》2015年01期 　论文类型：期刊论文

【摘要】：目的研究Stat1蛋白对宫颈癌Hela细胞生长、增殖及顺铂敏感性的影响及其作用机制。方法采用蛋白印迹法(Western blot)检测不同浓度DDP处理Hela细胞后Stat1蛋白表达差异;转染Stat1-si RNA后,通过Western blot验证干扰效果;应用四甲基偶氮唑蓝(MTT)法和5-溴脱氧尿嘧啶核苷(Brd U)观察转染Stat1-si RNA后Hela细胞对DDP敏感性变化,并检测Stat1相关细胞因子c-Myc的表达变化。结果随着DDP浓度的增加,Hela细胞中Stat1蛋白的表达量逐渐增加,DDP浓度为5 mg/L时,Stat1蛋白表达上调1.5倍,DDP浓度为10 mg/L时,Stat1蛋白表达上调近2倍;特异性Stat1-si RNA使Hela细胞中Stat1的表达下调70%,促进细胞生长和增殖;Stat1-si RNA可以降低DDP对Hela细胞生长和增殖的抑制效应,削弱DDP对c-Myc的抑制作用。结论 Hela细胞中c-Myc是STAT1发挥抑癌作用的靶点基因之一;STAT1/c-Myc通路介导了DDP抑制Hela细胞生长、增殖的作用;STAT1可增强Hela细胞对DDP的敏感性。
[Abstract]:Objective to study the effect of Stat1 protein on the growth of cervical cancer Hela cells. The effect and mechanism of proliferation and cisplatin sensitivity. Methods Western blot was used to detect the proliferation and cisplatin sensitivity. After Hela cells were treated with different concentrations of DDP, the expression of Stat1 protein was detected. After transfection of Stat1-si RNA, the interference effect was verified by Western blot. The sensitivity of Hela cells to DDP after transfection of Stat1-si RNA was observed by using tetramethyl azolyl azolium (MTT) and 5-bromodeoxyuridine (Brd UU). The expression of c-Myc, a cytokine associated with Stat1, was detected. Results with the increase of DDP concentration, the expression of Stat1 protein in Hela cells increased gradually. When the concentration of DDP was 5 mg/L, the expression of nongStat1 protein increased by 1. 5 times and increased by nearly 2 times when the concentration of DDP was 10 mg/L. Specific Stat1-si RNA down-regulated the expression of Stat1 in Hela cells and promoted the growth and proliferation of Hela cells. Stat1-si RNA could reduce the inhibitory effect of DDP on the growth and proliferation of Hela cells. Conclusion c-Myc is one of the target genes of STAT1 in Hela cells. STAT1/c-Myc pathway mediates the inhibitory effect of DDP on the growth and proliferation of Hela cells. STAT1 could enhance the sensitivity of Hela cells to DDP.
【作者单位】：中南大学湘雅三医院妇科;
【基金】：国家自然科学青年科学基金(81402270) 湖南省科技厅重点项目(2012FJ2014)~~
【分类号】：R737.33
【正文快照】： 宫颈癌是全球女性最常见的恶性肿瘤之一,发病率仅次于乳腺癌[1]。顺铂(cisplatin,DDP)广泛应用于恶性肿瘤的化学治疗,宫颈癌新辅助化疗方案多以顺铂为基础,疗效肯定。但顺铂的毒性反应突出,主要为肾功能损害、耳毒性、血液毒性、胃肠道反应等,寻找一类安全可靠的可增强肿瘤化

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